Department of Epidemiology and Biostatistics, College of Public Health, Zhengzhou University, Zhengzhou, China.
Department of Occupational Health and Occupational Diseases, College of Public Health, Zhengzhou University, Zhengzhou, China.
Front Public Health. 2022 Jul 5;10:904856. doi: 10.3389/fpubh.2022.904856. eCollection 2022.
The mitochondrial DNA (mtDNA) copy number is a vital component in maintaining normal mitochondrial function. It is affected by environmental and occupational exposures, as well as polymorphisms in nuclear genes. Nonetheless, the specific roles of polymorphisms in cell-cycle genes and mtDNA copy number are still unknown. This study enrolled a sample of 544 coke oven workers and 238 non-exposed controls so as to assess the effect of exposure of coke oven emissions (COEs) and polymorphisms in cell-cycle genes on the mtDNA copy number. We found that the mtDNA copy number in the exposed group (0.60 ± 0.29) was significantly lower than that in the control group (1.03 ± 0.31) ( =18.931, < 0.001). The analysis of covariance showed that both the rs1801270 (CA+CC) and the rs1059234 (CT+CC) in gene were associated with lower mtDNA copy number in the exposed group ( = 0.001). Generalized linear models indicated COEs-exposure (β = -0.432, < 0.001) and rs1059234 (CT+CC) in gene (β = -0.060, = 0.024) were the factors in mtDNA copy number reduction. In conclusion, this study suggests that the decrease of the mtDNA copy number is associated with COEs-exposure and the rs1059234 (CT+CC) in the gene.
线粒体 DNA（mtDNA）拷贝数是维持正常线粒体功能的重要组成部分。它受到环境和职业暴露以及核基因多态性的影响。然而，细胞周期基因多态性和 mtDNA 拷贝数的具体作用仍不清楚。本研究纳入了 544 名焦炉工人和 238 名非暴露对照者的样本，以评估焦炉排放物（COE）暴露和细胞周期基因多态性对 mtDNA 拷贝数的影响。我们发现暴露组的 mtDNA 拷贝数（0.60±0.29）明显低于对照组（1.03±0.31）（=18.931，<0.001）。协方差分析表明，基因中的 rs1801270（CA+CC）和 rs1059234（CT+CC）均与暴露组 mtDNA 拷贝数降低有关（=0.001）。广义线性模型表明，COE 暴露（β=-0.432，<0.001）和基因中的 rs1059234（CT+CC）（β=-0.060，=0.024）是 mtDNA 拷贝数减少的因素。总之，本研究表明 mtDNA 拷贝数的减少与 COE 暴露和基因中的 rs1059234（CT+CC）有关。
