Smeaton I, Summers C W, Harris R, Strachan T
Immunogenetics. 1987;25(3):179-83. doi: 10.1007/BF00344032.
We have used the HLA-C-specific DNA probe pC250 to investigate restriction fragment length polymorphism (RFLP) at the HLA-C locus. Genomic Southern blot hybridization included DNA prepared from a panel of homozygous typing cells representing serological specificities Cw1 to Cw8 and also from samples representing Cw blanks. Although many restriction nucleases failed to reveal any polymorphism, RFLPs were evident with Taq I, Pvu II, Bst XI, Nde I, and Nci I in addition to the previously reported Eco RI. In the case of Bst XI, a unique RFLP defined a subset of serologically defined Cw blanks. Comparison of RFLP sizes with restriction fragment lengths obtained from the known HLA-Cw3 gene sequence permitted the localization of intragenic C locus RFLPs and the identification of a variable Taq I site in the second intron, a variable Nci I site near the end of the fourth exon, and a variable Pvu II site in the fifth intron.
我们使用了HLA - C特异性DNA探针pC250来研究HLA - C位点的限制性片段长度多态性（RFLP）。基因组Southern印迹杂交使用的DNA，一部分来自代表血清学特异性Cw1至Cw8的纯合分型细胞系，另一部分来自代表Cw空白的样本。尽管许多限制性核酸酶未能揭示任何多态性，但除了先前报道的Eco RI外，Taq I、Pvu II、Bst XI、Nde I和Nci I也显示出明显的RFLP。就Bst XI而言，一种独特的RFLP定义了血清学定义的Cw空白的一个子集。将RFLP大小与从已知HLA - Cw3基因序列获得的限制性片段长度进行比较，从而确定了基因内C位点RFLP的定位，并鉴定出第二个内含子中的一个可变Taq I位点、第四个外显子末端附近的一个可变Nci I位点以及第五个内含子中的一个可变Pvu II位点。
