Koller B H, Sidwell B, DeMars R, Orr H T
Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175-8. doi: 10.1073/pnas.81.16.5175.
When human class I cDNA clones containing coding sequences are used to probe genomic DNA, 15-20 fragments, each containing a complete class I gene or pseudogene, are seen. Identification of which genomic DNA segments encode the HLA-A and -B antigens has to date required transfection of mouse L cells with cloned class I genes or analysis of HLA loss mutants. In this report we show that under high-stringency conditions, probes constructed from the 3'-untranslated region can be used to specifically identify the segments of DNA that encode the HLA-A and -B antigens in the human lymphoblastoid cell line 721. Examination of DNA from unrelated individuals indicates that these probes are locus specific and will permit identification of HLA-A and -B genes in the population.
当使用含有编码序列的人类I类cDNA克隆来探测基因组DNA时，可以看到15 - 20个片段，每个片段都包含一个完整的I类基因或假基因。迄今为止，要确定哪些基因组DNA片段编码HLA - A和 - B抗原，需要用克隆的I类基因转染小鼠L细胞或分析HLA缺失突变体。在本报告中，我们表明在高严格条件下，由3'非翻译区构建的探针可用于特异性鉴定人类淋巴母细胞系721中编码HLA - A和 - B抗原的DNA片段。对无关个体的DNA检测表明，这些探针具有位点特异性，能够在群体中鉴定HLA - A和 - B基因。
