Department of Animal Sciences, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea.
State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Insititute of Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
Sci Rep. 2017 Aug 17;7(1):8632. doi: 10.1038/s41598-017-09282-2.
Bisphenol A (BPA) is an environmental contaminant widely used in the plastic industry. BPA has been demonstrated to be an endocrine disruptor and has an adverse effect on the embryonic development of mammals. However, the mechanism of action of BPA is limited. In this study, we investigated the role and mechanism of BPA in porcine embryonic development. First, the parthenotes were treated with different concentrations of BPA. We found that blastocyst formation was impaired and the parthenotes were arrested at the 4-cell stage after treatment with 100 μm BPA. Second, ROS increased following the addition of BPA, which further caused mitochondrial damage, and cytochrome c was released from the mitochondria to induce apoptosis. The adaptive response was demonstrated through LC3 immunofluorescence staining and by assessing autophagy-related gene expression. In addition, BPA caused DNA damage through the p53-p21 signaling pathway. Thus, our results indicate that BPA displays an adverse effect on porcine early embryonic development through mitochondrial and DNA damage.
双酚 A（BPA）是一种广泛应用于塑料工业的环境污染物。BPA 已被证明是一种内分泌干扰物，对哺乳动物的胚胎发育有不良影响。然而，BPA 的作用机制有限。在这项研究中，我们研究了 BPA 在猪胚胎发育中的作用和机制。首先，用不同浓度的 BPA 处理孤雌原核。我们发现，当用 100μm BPA 处理时，囊胚形成受损，孤雌原核在 4 细胞期停滞。其次，加入 BPA 后 ROS 增加，进而导致线粒体损伤，细胞色素 c 从线粒体释放，引发细胞凋亡。通过 LC3 免疫荧光染色和评估自噬相关基因表达，观察到适应性反应。此外，BPA 通过 p53-p21 信号通路导致 DNA 损伤。因此，我们的结果表明，BPA 通过线粒体和 DNA 损伤对猪早期胚胎发育产生不良影响。
