Ferris Jacqueline, Favetta Laura A, King W Allan
Department of Biomedical Sciences, University of Guelph, Guelph, Ont., Canada.
Cytogenet Genome Res. 2015;145(1):50-8. doi: 10.1159/000381321. Epub 2015 Apr 11.
Bisphenol A (BPA) exposure in humans is widespread, and BPA has been detected in a variety of samples including follicular fluid. BPA levels have been found to negatively correlate with the developmental potential of oocytes in women undergoing in vitro fertilization and to induce meiotic abnormalities experimentally in human and mouse models. BPA may detrimentally affect oocyte maturation, and different concentrations of exposure can cause various outcomes. Because of the importance of oocyte maturation on developmental potential, disturbances during this time can significantly impact oocyte viability. Here, bovine oocytes were matured in vitro with and without BPA treatment of the media. The levels of BPA taken up by the oocytes were much lower than the initial exposure. Medium treatment with 30 ng/ml resulted in an average of 2.48 ng/ml BPA measured in mature oocytes. These oocytes exhibited decreased maturation and increased incidence of spindle abnormalities. Only 57.4% of oocytes exposed to 30 ng/ml BPA reached maturity compared to 72.4% of controls (p < 0.05). Mature oocytes following BPA exposure displayed increased abnormal spindle morphology (67.9%) and chromosome dispersal (60%) compared to all other groups analyzed (p < 0.05). Thus, exposure to BPA during in vitro oocyte maturation has the potential to decrease oocyte quality.
双酚A（BPA）在人类中的暴露十分普遍，并且在包括卵泡液在内的各种样本中都检测到了BPA。已发现BPA水平与接受体外受精的女性卵母细胞的发育潜能呈负相关，并且在人类和小鼠模型中通过实验诱导减数分裂异常。BPA可能会对卵母细胞成熟产生不利影响，不同浓度的暴露会导致各种结果。由于卵母细胞成熟对发育潜能的重要性，在此期间的干扰会显著影响卵母细胞的活力。在此，将牛卵母细胞在添加和不添加BPA的培养基中进行体外成熟培养。卵母细胞摄取的BPA水平远低于初始暴露水平。用30 ng/ml处理培养基后，成熟卵母细胞中测得的BPA平均水平为2.48 ng/ml。这些卵母细胞表现出成熟度降低和纺锤体异常发生率增加。与72.4%的对照组相比，仅57.4%暴露于30 ng/ml BPA的卵母细胞达到成熟（p<0.05）。与所有其他分析组相比，BPA暴露后的成熟卵母细胞显示出异常纺锤体形态（67.9%）和染色体分散（60%）增加（p<0.05）。因此，在体外卵母细胞成熟过程中暴露于BPA有可能降低卵母细胞质量。
