Stinson R S, Lemmon R D, Baggett D W, Huff T L, Malone J A, Sloan G L, Winters A L
J Biol Stand. 1986 Oct;14(4):261-71. doi: 10.1016/0092-1157(86)90014-4.
Protective antigen was extracted from Bordetella pertussis cells with 1.0 M NaCl and precipitated with ammonium sulfate, 20-40% saturation (designated fraction 15A-1B). The protective antigen was purified further by detergent (Emulphogene BC720) treatment and adsorption to aluminum hydroxide gel (designated fraction 15A-108A). Compared with B. pertussis vaccine and fraction 15A-1B, fraction 15A-108A retained protective activity as assessed by the mouse protection test, but had reduced protein and markedly reduced endotoxin content. Fraction 15A-108A also had reduced leukocytosis-promoting, histamine sensitizing splenomegaly-inducing, and adjuvant activities. Emulphogene treatment provided a relatively simple method for removing endotoxin from a potential acellular B. pertussis vaccine.
用1.0 M氯化钠从百日咳博德特氏菌细胞中提取保护性抗原，并用饱和度为20 - 40%的硫酸铵沉淀（命名为15A - 1B组分）。通过去污剂（乳化剂BC720）处理并吸附到氢氧化铝凝胶上进一步纯化保护性抗原（命名为15A - 108A组分）。与百日咳博德特氏菌疫苗和15A - 1B组分相比，通过小鼠保护试验评估，15A - 108A组分保留了保护活性，但蛋白质含量降低，内毒素含量显著降低。15A - 108A组分还具有降低的促白细胞增多、组胺致敏、脾肿大诱导和佐剂活性。乳化剂处理为从潜在的无细胞百日咳博德特氏菌疫苗中去除内毒素提供了一种相对简单的方法。
