Zakharova N S, Remova T N, Bazhanova I G, Ozeretskovskaia M N, Shmeleva E I
Zh Mikrobiol Epidemiol Immunobiol. 1987 Jun(6):38-43.
The biological properties of Bordetella pertussis antigenic complex, obtained by a technologically simple method from the medium used for the cultivation of B. pertussis, were studied. The preparation was characterized by pronounced hemagglutinating activity, toxicity, histamine-sensitizing and leukocytosis-stimulating activity and produced a cytopathogenic effect on the culture of Chinese hamster ovary cells. The detoxified preparations showed pronounced protective activity in experiments on the active and passive protection of mice. The ED50 of the preparation was 0.146 microgram of protein. In the proposed human immunization dose containing 10 micrograms of protein the detoxified preparation showed no hemagglutinating, leukocytosis-stimulating or histamine-sensitizing activity and proved to be nontoxic in the weight loss test on mice.
研究了通过一种技术简单的方法从用于培养百日咳博德特氏菌的培养基中获得的百日咳博德特氏菌抗原复合物的生物学特性。该制剂具有明显的血凝活性、毒性、组胺致敏和白细胞增多刺激活性，并对中国仓鼠卵巢细胞培养物产生细胞致病作用。在对小鼠进行主动和被动保护的实验中，解毒制剂显示出明显的保护活性。该制剂的半数有效剂量（ED50）为0.146微克蛋白质。在所提议的含10微克蛋白质的人体免疫剂量中，解毒制剂未显示出血凝、白细胞增多刺激或组胺致敏活性，并且在小鼠体重减轻试验中证明无毒。
