Megret F, Fournier J M, Alouf J E
Med Microbiol Immunol. 1984;173(4):225-32. doi: 10.1007/BF02122114.
Bordetella pertussis bacterial cells, bacterial extracts, and concentrated culture supernatant fluid were comparatively examined for histamine sensitizing and leucocytosis promoting activities, toxicity (mouse weight gain test), immunoprotective potency and lipopolysaccharide bioassay. The activity of histamine sensitizing factor always paralleled that of leucocytosis promoting factor. In contrast, important differences were demonstrated regarding the toxicity and protective activity of the three preparations. Culture supernatant was more toxic and less protective than either bacterial cells or cell extract. Although the latter had lower protective potency than whole cells, its lower toxicity might lead to its consideration as a possible potential vaccine.
对百日咳博德特氏菌的细菌细胞、细菌提取物和浓缩培养上清液进行了组胺致敏活性、促白细胞增多活性、毒性（小鼠体重增加试验）、免疫保护效力和脂多糖生物测定的比较研究。组胺致敏因子的活性总是与促白细胞增多因子的活性平行。相比之下，三种制剂在毒性和保护活性方面表现出重要差异。培养上清液比细菌细胞或细胞提取物毒性更大，保护性更弱。虽然细胞提取物的保护效力低于全细胞，但其较低的毒性可能使其被视为一种潜在的疫苗。
