Iida T, Horiuchi Y
J Biol Stand. 1987 Jan;15(1):17-26. doi: 10.1016/0092-1157(87)90013-8.
To improve the whole-cell pertussis vaccine we have studied the inactivation of the biological properties characteristic of Bordetella pertussis phase I bacteria, i.e. histamine-sensitizing, lymphocytosis-promoting and mouse protective activities, by treating a concentrated bacterial suspension with various concentrations of glutaraldehyde. Under the experimental conditions, treatment with 10 mM glutaraldehyde at 37 degrees C for 30 min resulted in a marked reduction of the toxic activities without grossly diminishing the protective potency. Further tests were performed on the stability of the protective potency, on the agglutinin production in mice, and on the freedom from abnormal toxicity in guinea-pigs.
为改进全细胞百日咳疫苗，我们研究了用不同浓度的戊二醛处理百日咳博德特氏菌I相细菌浓缩悬液，以使其具有的生物学特性（即组胺致敏、淋巴细胞增多促进和小鼠保护活性）失活。在实验条件下，37℃用10 mM戊二醛处理30分钟可使毒性活性显著降低，而不会大幅降低保护效力。还对保护效力的稳定性、小鼠中凝集素的产生以及豚鼠无异常毒性进行了进一步测试。
