Park Sooyeon, Choi Su Jung, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Cheoncheon-dong 300, Jangan-gu, Suwon 440-746, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2959-2963. doi: 10.1099/ijsem.0.002056. Epub 2017 Aug 18.
A Gram-stain-negative, non-motile, aerobic and ovoid or rod-shaped bacterium, designated SCTF-16T, was isolated from a tidal flat on the Yellow Sea, South Korea. Strain SCTF-16T grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 2.0 % (w/v) NaCl. In the neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences, strain SCTF-16T clustered with the type strains of two species of the genus Umboniibacter. Strain SCTF-16T exhibited 16S rRNA gene sequence similarities of 99.6 and 97.1 % to the type strains of Umboniibacterroseus and Umboniibactermarinipuniceus, respectively, and of less than 93.4 % to the type strains of the other recognized species. Strain SCTF-16T contained Q-7 as the predominant ubiquinone and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C13 : 0 as the major fatty acids. The major polar lipids detected in strain SCTF-16T were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content of strain SCTF-16T was 50.0 mol%, and its mean DNA-DNA relatedness values with the type strains of U. roseus and U. marinipuniceus were 31 and 9 %, respectively. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain SCTF-16T is separated from the two recognized species of the genus Umboniibacter. On the basis of the data presented, strain SCTF-16T is considered to represent a novel species of the genus Umboniibacter, for which the name Umboniibacter caenipelagi sp. nov. is proposed. The type strain is SCTF-16T (=KCTC 52628T=NBRC 112760T).
从韩国黄海潮间带分离出一株革兰氏染色阴性、无运动性、需氧的卵形或杆状细菌，命名为SCTF-16T。菌株SCTF-16T在30 °C、pH 7.0 - 7.5以及2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法、最大似然法和最大简约法系统发育树中，菌株SCTF-16T与Umboniibacter属两个种的模式菌株聚类在一起。菌株SCTF-16T与Umboniibacter roseus和Umboniibacter marinopuniceus模式菌株的16S rRNA基因序列相似性分别为99.6%和97.1 %，与其他已确认种的模式菌株相似性均低于93.4 %。菌株SCTF-16T以Q-7作为主要泛醌，以特征性总和3（C16 : 1ω7c和/或C16 : 1ω6c）和C13 : 0作为主要脂肪酸。在菌株SCTF-16T中检测到的主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。菌株SCTF-16T的DNA G+C含量为50.0 mol%，其与U. roseus和U. marinopuniceus模式菌株的平均DNA-DNA相关性值分别为31%和9 %。这些差异表型特征，连同系统发育和遗传独特性，表明菌株SCTF-16T与Umboniibacter属已确认的两个种不同。基于所提供的数据，菌株SCTF-16T被认为代表Umboniibacter属的一个新种，为此提议命名为Umboniibacter caenipelagi sp. nov.。模式菌株为SCTF-16T（=KCTC 52628T=NBRC 112760T）。
