Park Sooyeon, Choi Su Jung, Park Ji-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2791-2797. doi: 10.1099/ijsem.0.002023. Epub 2017 Aug 18.
A Gram-stain-negative, aerobic, non-spore-forming, motile and ovoid or rod-shaped bacterial strain, JDTF-113T, was isolated from a tidal flat in Jindo, an island of South Korea. Strain JDTF-113T grew optimally at 30 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. A neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences, revealed that strain JDTF-113T fell within the clade enclosing the type strains of species of the genus Alteromonas. Strain JDTF-113T exhibited 16S rRNA gene sequence similarity values of 97.1-98.1 % to the type strains of Alteromonaslipolytica, Alteromonaslitorea, Alteromonasmediterranea, Alteromonasconfluentis, Alteromonas hispanica, Alteromonasgenovensis and Alteromonasmarina, and of 94.8-96.9 % to those of the other species of the genus Alteromonas. Strain JDTF-113T contained Q-8 as the predominant ubiquinone and C16 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and C18 : 1ω7c as the major fatty acids. The major polar lipids of strain JDTF-113T were phosphatidylethanolamine, phosphatidylglycerol and one unidentified glycolipid. The DNA G+C content of strain JDTF-113T was 51.1 mol% and its mean DNA-DNA relatedness values with the type strains of seven closely phylogenetically related species of the genus Alteromonaswere was 10-23 %. The differential phenotypic properties and phylogenetic and genetic distinctiveness support strain JDTF-113T being separated from species of the genus Alteromonaswith validly publishednames. On the basis of the data presented, strain JDTF-113T is considered to represent a novel species of the genus Alteromonas, for which the name Alteromonas aestuariivivens sp. nov. is proposed. The type strain is JDTF-113T (=KCTC 52655T=NBRC 112708T).
从韩国珍岛的潮滩中分离出一株革兰氏染色阴性、需氧、无芽孢形成、具运动性且呈卵形或杆状的细菌菌株JDTF-113T。菌株JDTF-113T在30 °C、pH 7.0 - 8.0以及2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树显示，菌株JDTF-113T位于包含交替单胞菌属各物种模式菌株的进化枝内。菌株JDTF-113T与解脂交替单胞菌、滨岸交替单胞菌、地中海交替单胞菌、融合交替单胞菌、西班牙交替单胞菌、热那亚交替单胞菌和海生交替单胞菌的模式菌株的16S rRNA基因序列相似性值为97.1 - 98.1 %，与交替单胞菌属其他物种的模式菌株的相似性值为94.8 - 96.9 %。菌株JDTF-113T以Q-8作为主要泛醌，以C16 : 0、总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）以及C18 : 1ω7c作为主要脂肪酸。菌株JDTF-113T的主要极性脂类为磷脂酰乙醇胺磷脂酰甘油和一种未鉴定的糖脂。菌株JDTF-113T的DNA G+C含量为51.1 mol%，其与交替单胞菌属七个系统发育关系密切的相关物种的模式菌株的平均DNA - DNA相关性值为10 - 23 %。其不同的表型特征以及系统发育和遗传独特性支持菌株JDTF-113T与已有效发表名称的交替单胞菌属物种区分开来。基于所呈现的数据，菌株JDTF-113T被认为代表交替单胞菌属的一个新物种，为此提议将其命名为河口交替单胞菌（Alteromonas aestuariivivens sp. nov.）。模式菌株为JDTF-113T（=KCTC 52655T=NBRC 112708T）。
