Park Sooyeon, Jung Yong-Taek, Kang Chul-Hyung, Park Ja-Min, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3676-3682. doi: 10.1099/ijs.0.067611-0. Epub 2014 Aug 1.
A Gram-stain-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated GJSW-36(T), was isolated from seawater at Geoje island in the South Sea, South Korea. Strain GJSW-36(T) grew optimally at pH 7.0-8.0, at 25 °C and in the presence of 2% (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GJSW-36(T) fell within the clade comprising the type strains of species of the genus Thalassotalea and Thalassomonas fusca. Strain GJSW-36(T) exhibited 16S rRNA gene sequence similarity values of 94.2-96.0% to the type strains of species of the genus Thalassotalea and Thalassomonas fusca and of 93.8-94.5% to the type strains of the other species of the genus Thalassomonas. Strain GJSW-36(T) contained ubiquinone-8 (Q-8) as the predominant ubiquinone and summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c), C(1:1)ω8c and C(16:0) as the major fatty acids. The major polar lipids of strain GJSW-36(T) were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain GJSW-36(T) was 45.1 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GJSW-36(T) is separated from species of the genus Thalassotalea and Thalassomonas fusca. On the basis of the data presented, strain GJSW-36(T) is considered to represent a novel species of the genus Thalassotalea, for which the name Thalassotalea ponticola sp. nov. is proposed. The type strain is GJSW-36(T) ( =KCTC 42155(T) =CECT 8656(T)). From this study, it is also proposed that Thalassomonas fusca should be reclassified as a member of the genus Thalassotalea and the description of the genus Thalassotalea is emended.
从韩国南海巨济岛的海水中分离出一株革兰氏阴性、需氧、无鞭毛、杆状或卵形的细菌菌株，命名为GJSW-36(T)。菌株GJSW-36(T)在pH 7.0-8.0、25℃和2%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的邻接法系统发育树表明，菌株GJSW-36(T)属于包含嗜盐海杆菌属（Thalassotalea）各物种模式菌株和褐藻海单胞菌（Thalassomonas fusca）的进化枝。菌株GJSW-36(T)与嗜盐海杆菌属各物种模式菌株和褐藻海单胞菌的16S rRNA基因序列相似性值为94.2-96.0%，与海单胞菌属其他物种模式菌株的相似性值为93.8-94.5%。菌株GJSW-36(T)以泛醌-8（Q-8）作为主要泛醌，主要脂肪酸为特征性总和3（C(16:1)ω7c和/或C(16:1)ω6c）、C(18:1)ω8c和C(16:0)。菌株GJSW-36(T)的主要极性脂为磷脂酰甘油和磷脂酰乙醇胺。菌株GJSW-36(T)的DNA G+C含量为45.1 mol%。不同的表型特征以及系统发育独特性表明，菌株GJSW-36(T)与嗜盐海杆菌属和褐藻海单胞菌的物种不同。根据所提供的数据，菌株GJSW-36(T)被认为代表嗜盐海杆菌属的一个新物种，为此提出新名称嗜盐海杆菌海桥种（Thalassotalea ponticola sp. nov.）。模式菌株为GJSW-36(T)（=KCTC 42155(T)=CECT 8656(T)）。基于本研究，还建议将褐藻海单胞菌重新分类为嗜盐海杆菌属的成员，并对嗜盐海杆菌属的描述进行修订。
