Yan Zheng-Fei, Lin Pei, Li Chang-Tian, Kook MooChang, Wang Qi-Jun, Yi Tae-Hoo
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2873-2878. doi: 10.1099/ijsem.0.002036. Epub 2017 Aug 18.
A Gram-stain-negative, aerobic, non-motile and coccoid to short-rod-shaped bacterial strain (THG-N2.22T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 20-40 °C (optimum 28 °C), at pH 5-9 (optimum 7) and with 0-4 % (w/v) NaCl (optimum 1 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-N2.22T were identified as Roseomonas rhizosphaerae YW11T (98.5 % similarity), Roseomonas rubra S5T (98.5 %), Roseomonas cervicalis ATCC 49957T (98.2 %), Roseomonas aestuarii JC17T (97.8 %), Roseomonas oryzae JC288T (97.3 %) and Roseomonas ludipueritiae 170/96T (97.3 %); levels of similarity with the type strains of other Roseomonas species were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, two unidentified aminolipids, three unidentified phospholipids and three unidentified lipids. The major quinone was ubiquinone-10. The major fatty acids were C16 : 0, C18 : 1 2-OH, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The DNA G+C content of strain THG-N2.22T was 64.1 mol%. DNA-DNA hybridization values between strain THG-N2.22T and R. rhizosphaerae YW11T, R. rubra S5T, R. cervicalis ATCC 49957T, R. aestuarii JC17T, R. oryzae JC288T and R. ludipueritiae 170/96T were 43.1 % (30.2 %, reciprocal analysis), 39.0 % (24.7 %), 34.4 % (15.2 %), 18.0 % (14.5 %), 14.7 % (9.7 %) and 11.0 % (5.6 %), respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-N2.22T represents a novel species of the genus Roseomonas, for which the name Roseomonas hibiscisoli sp. nov. is proposed. The type strain is THG-N2.22T (=KACC 18935T=CCTCC AB 2016176T).
从木槿（ Hibiscus syriacus ）根际分离出一株革兰氏染色阴性、需氧、无运动性、球状至短杆状的细菌菌株（THG-N2.22T）。该菌株在20-40°C（最适温度28°C）、pH 5-9（最适pH 7）和0-4%（w/v）NaCl（最适1%）条件下生长。基于16S rRNA基因序列分析，菌株THG-N2.22T最近的系统发育邻居被鉴定为根际玫瑰单胞菌YW11T（相似度98.5%）、红色玫瑰单胞菌S5T（98.5%）、宫颈玫瑰单胞菌ATCC 49957T（98.2%）、河口玫瑰单胞菌JC17T（97.8%）、稻玫瑰单胞菌JC288T（97.3%）和戏谑玫瑰单胞菌170/96T（97.3%）；与其他玫瑰单胞菌属模式菌株的相似度低于97.0%。极性脂质为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、两种未鉴定的氨基脂质、三种未鉴定的磷脂和三种未鉴定的脂质。主要醌为泛醌-10。主要脂肪酸为C16:0、C18:1 2-OH、C18:1ω7c和总和特征3（C16:1ω7c和/或C16:1ω6c）。菌株THG-N2.22T的DNA G+C含量为64.1 mol%。菌株THG-N2.22T与根际玫瑰单胞菌YW11T、红色玫瑰单胞菌S5T、宫颈玫瑰单胞菌ATCC 49957T、河口玫瑰单胞菌JC17T、稻玫瑰单胞菌JC288T和戏谑玫瑰单胞菌170/96T之间的DNA-DNA杂交值分别为43.1%（相互分析为30.2%）、39.0%（24.7%）、34.4%（15.2%）、18.0%（14.5%）、14.7%（9.7%）和11.0%（5.6%）。基于系统发育分析、化学分类数据、生理特征和DNA-DNA杂交数据，菌株THG-N2.22T代表玫瑰单胞菌属的一个新物种，为此提出名为木槿玫瑰单胞菌（Roseomonas hibiscisoli sp. nov.）的新名称。模式菌株为THG-N2.22T（=KACC 18935T=CCTCC AB 2016176T）。
