Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do, 17104, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Int J Syst Evol Microbiol. 2017 Oct;67(10):3773-3777. doi: 10.1099/ijsem.0.002191. Epub 2017 Sep 7.
A Gram-negative, aerobic, non-motile, long rods or coccoid without flagellum strain, designated THG-YS3.2.7, was isolated from therhizosphere soil of a Mugunghwa flower collected from Kyung Hee University, Yongin, South Korea. Growth occurred at 10-40 °C (optimum 28-37 °C), at pH 6-8 (optimum 7) and at 0-5 % NaCl (optimum 1 %). The predominant ubiquinone was ubiquinone 8 (Q-8). The major cellular fatty acids were C10 : 0, C10 : 0 3OH, C16 : 0, C17 : 0, C17 : 0 cyclo, C18 : 0, C18 : 3ω6c (6,9,12), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1ω6c). The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylcholine (PC). The DNA G+C content of strain THG-YS3.2.7 was 69.4 mol%. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-YS3.2.7 were identified as Ramlibacter henchirensis DSM 14656 (97.92 %), Ramlibacter tataouinensis DSM 14655 (97.90 %), Ramlibacter solisilvae KACC 17567 (97.04 %). DNA-DNA hybridization values between strain THG-YS3.2.7 and R. henchirensis DSM 14656, R. tataouinensis DSM 14655, R. solisilvae KACC 17567 were 32.5±1.5, 43.1±1.1, 42.8±1.1 %, respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-YS3.2.7 represent a novel species of the genus Ramlibacter, for which the name Ramlibacter rhizophilus sp. nov. is proposed. The type strain is THG-YS3.2.7 (=KCTC 52083=CCTCC AB 2015357).
一株革兰氏阴性、需氧、非运动、长杆或球菌状、无鞭毛的菌株，命名为 THG-YS3.2.7，从韩国庆熙大学永进校区采集的木槿花根际土壤中分离得到。该菌在 10-40°C（最适 28-37°C）、pH 值 6-8（最适 7）和 0-5%NaCl（最适 1%）条件下生长。主要的醌类为泛醌 8（Q-8）。主要的细胞脂肪酸为 C10:0、C10:0 3OH、C16:0、C17:0、C17:0 环、C18:0、C18:3ω6c（6,9,12）、特征性脂肪酸 3（C16:1ω7c 和/或 C16:1ω6c）和特征性脂肪酸 8（C18:1 ω7c 和/或 C18:1ω6c）。主要的极性脂类为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）、磷脂酰乙醇胺（PE）、磷脂酰胆碱（PC）。菌株 THG-YS3.2.7 的 DNA G+C 含量为 69.4 mol%。根据 16S rRNA 基因序列分析，菌株 THG-YS3.2.7 的最接近的系统发育亲缘关系的菌株为亨氏拉姆菌 DSM 14656（97.92%）、塔塔乌因氏拉姆菌 DSM 14655（97.90%）、索利斯氏拉姆菌 KACC 17567（97.04%）。菌株 THG-YS3.2.7 与亨氏拉姆菌 DSM 14656、塔塔乌因氏拉姆菌 DSM 14655、索利斯氏拉姆菌 KACC 17567 的 DNA-DNA 杂交值分别为 32.5±1.5、43.1±1.1、42.8±1.1%。基于系统发育分析、化学分类学数据、生理特性和 DNA-DNA 杂交数据，菌株 THG-YS3.2.7 代表拉姆菌属的一个新种，命名为根际拉姆菌 Ramlibacter rhizophilus sp. nov.。模式菌株为 THG-YS3.2.7（=KCTC 52083=CCTCC AB 2015357）。
