Lin Pei, Yan Zheng-Fei, Won Kyung-Hwa, Yang Jung-Eun, Li Chang-Tian, Kook MooChang, Wang Qi-Jun, Yi Tae-Hoo
Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdae-ro, Giheung-gu, Yongin-si, Gyeonggi-do 17104, Republic of Korea.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, PR China.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2452-2458. doi: 10.1099/ijsem.0.001990. Epub 2017 Jul 26.
A Gram-reaction-negative, aerobic, non-motile, short-rod-shaped bacterium (THG-T2.31T) was isolated from the rhizosphere of Mugunghwa (Hibiscus syriacus). Growth occurred at 10-35 °C (optimum 28 °C), at pH 5.0-8.0 (optimum pH 7.0) and with 0-4.0 % NaCl (optimum 1.0 %). Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbours of strain THG-T2.31T were identified as Paracoccus marcusii DSM 11574T (98.4 %), Paracoccus haeundaensis BC74171T (98.3 %), Paracoccus carotinifaciens E-396T (98.3 %), Paracoccus aestuarii B7T (97.3 %) and Paracoccus seriniphilus MBT-A4T (97.0 %); levels of similarity with the type strains of other species of the genus Paracoccus were lower than 97.0 %. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified aminolipid and two unidentified phospholipids. The major fatty acids were C16 : 0, C18 : 0, C10 : 0 3-OH, and C18 : 1ω7c. The quinone was ubiquinone-10 (Q-10). The DNA G+C content of strain THG-T2.31T was 69.1 mol%. DNA-DNA hybridization values between strain THG-T2.31T and P. marcusii DSM 11574T, P. haeundaensis BC74171T, P. carotinifaciens E-396T, P. aestuarii B7T and P. seriniphilus MBT-A4T were 38.9 % (34.9 %, reciprocal analysis), 29.1 % (23.5 %), 28.0 % (19.7 %), 18.9 % (9.3) and 13.1 % (6.2 %). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T2.31T represents a novel species of the genus Paracoccus, for which the name Paracoccus hibiscisoli sp. nov. is proposed. The type strain is THG-T2.31T (=KACC 18933T=CCTCC AB 2016182T).
从木槿（ Hibiscus syriacus ）根际分离出一株革兰氏阴性、需氧、无运动性、短杆状细菌（THG-T2.31T）。该菌在10-35 °C（最适温度28 °C）、pH 5.0-8.0（最适pH 7.0）和0-4.0 % NaCl（最适1.0 %）条件下生长。基于16S rRNA基因序列分析，菌株THG-T2.31T最近的系统发育邻种被鉴定为马库斯副球菌DSM 11574T（相似度98.4 %）、海州副球菌BC74171T（98.3 %）、产胡萝卜素副球菌E-396T（98.3 %）、河口副球菌B7T（97.3 %）和嗜丝氨酸副球菌MBT-A4T（97.0 %）；与副球菌属其他种模式菌株的相似度低于97.0 %。极性脂为双磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的氨基脂和两种未鉴定的磷脂。主要脂肪酸为C16 : 0、C18 : 0、C10 : 0 3-OH和C18 : 1ω7c。醌为泛醌-10（Q-10）。菌株THG-T2.31T的DNA G+C含量为69.1 mol%。菌株THG-T2.31T与马库斯副球菌DSM 11574T、海州副球菌BC74171T、产胡萝卜素副球菌E-396T、河口副球菌B7T和嗜丝氨酸副球菌MBT-A4T之间的DNA-DNA杂交值分别为38.9 %（反向分析为34.9 %）、29.1 %（23.5 %）、28.0 %（19.7 %）、18.9 %（9.3）和13.1 %（6.2 %）。基于系统发育分析、化学分类数据、生理特征和DNA-DNA杂交数据，菌株THG-T2.31T代表副球菌属的一个新物种，提议将其命名为木槿副球菌（ Paracoccus hibiscisoli ）新种。模式菌株为THG-T2.31T（=KACC 18933T=CCTCC AB 2016182T）。
