Dahal Ram Hari, Shim Dong Seop, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Innogene, Co., #301 Woolim E-biz Center1, 28, Digital-ro 33-gil, Guro-gu, Seoul 08337, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3043-3049. doi: 10.1099/ijsem.0.002078. Epub 2017 Aug 18.
During isolation of soil bacteria having antibacterial functions, an aerobic, Gram-stain-positive, oxidase-negative, catalase-positive bacterium, designated strain R434T, was isolated. Strain R434T showed antimicrobial activity against Propionibacterium acnes and Staphylococcus epidermidis and significant enzyme-inhibitory capability. The diagnostic diamino acid of the cell-wall peptidoglycan was meso-diaminopimelic acid, and the whole-cell sugars were galactose, arabinose and glucose. Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain R434T formed a lineage within the family Pseudonocardiaceae. Strain R434T showed highest sequence similarity with type strains of the genus Actinokineospora, including Actinokineospora guangxiensis Gk-6T (99.4 % sequence similarity), Actinokineospora soli YIM 75948T (98.5 %), Actinokineospora fastidiosa IMSNU 20054T (98.0 %), Actinokineospora cibodasensis ID03-0784T (97.9 %), Actinokineospora terrae IFO 15668T (97.6 %) and Actinokineospora auranticolor IFO 16518T (97.4 %). The predominant respiratory quinone of strain R434T was MK-9(H4). The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine and unidentified glycolipid. The major cellular fatty acids were iso-C16 : 0, iso-C16 : 1 H and C17 : 1ω6c. The DNA G+C content of strain R434T was 71.6 mol%. On the basis of phenotypic, genotypic, chemotaxonomic and phylogenetic analysis, strain R434T represents a novel species of the genus Actinokineospora, for which the name Actinokineospora acnipugnans sp. nov. is proposed. The type strain of 'Actinokineospora acnipugnans' is R434T (=KEMB 9005-403T=KACC 18904T=JCM 31557T).
在分离具有抗菌功能的土壤细菌过程中，分离出了一株需氧、革兰氏染色阳性、氧化酶阴性、过氧化氢酶阳性的细菌，命名为R434T菌株。R434T菌株对痤疮丙酸杆菌和表皮葡萄球菌具有抗菌活性，并具有显著的酶抑制能力。细胞壁肽聚糖的诊断性二氨基酸为内消旋二氨基庚二酸，全细胞糖为半乳糖、阿拉伯糖和葡萄糖。基于16S rRNA基因序列的系统发育分析表明，R434T菌株在假诺卡氏菌科内形成了一个分支。R434T菌株与放线嗜皮菌属模式菌株的序列相似性最高，包括广西放线嗜皮菌Gk-6T（序列相似性99.4%）、土壤放线嗜皮菌YIM 75948T（98.5%）、苛求放线嗜皮菌IMSNU 20054T（98.0%）、芝田放线嗜皮菌ID03-0784T（97.9%）、土生放线嗜皮菌IFO 15668T（97.6%）和橙黄放线嗜皮菌IFO 16518T（97.4%）。R434T菌株的主要呼吸醌为MK-9（H4）。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺和未鉴定的糖脂。主要细胞脂肪酸为异-C16 : 0、异-C16 : 1 H和C17 : 1ω6c。R434T菌株的DNA G+C含量为71.6 mol%。基于表型、基因型、化学分类和系统发育分析，R434T菌株代表放线嗜皮菌属的一个新物种，为此提出新名称痤疮拮抗性放线嗜皮菌（Actinokineospora acnipugnans sp. nov.）。“痤疮拮抗性放线嗜皮菌”的模式菌株为R434T（=KEMB 9005-403T=KACC 18904T=JCM 31557T）。
