Research Center for Biotechnology, Indonesian Institute of Sciences, Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia.
Department of Biotechnology, National Institute of Technology and Evaluation, 2-5-8 Kazusa kamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2010 Oct;60(Pt 10):2331-2335. doi: 10.1099/ijs.0.013276-0. Epub 2009 Nov 20.
Six actinomycete strains isolated from soil and plant-litter samples in Indonesia were studied for their taxonomic position by using a polyphasic approach. Phylogenetically, all the strains were located in the broad cluster of the genus Actinokineospora. Chemotaxonomic data [cell-wall diamino acid, meso-diaminopimelic acid; cell-wall peptidoglycan, type III (A1γ); major sugars, galactose and arabinose; major menaquinone, MK-9(H₄); major fatty acid, iso-C₁₆:₀; major phospholipid, phosphatidylethanolamine] supported the affiliation of all six strains to the genus Actinokineospora. The results of DNA-DNA hybridization with DNA from type strains of Actinokineospora species with validly published names revealed three DNA-DNA relatedness groups. Group I (ID03-0561(T)) showed low relatedness to the other strains studied. The three strains in group II (ID03-0784(T), ID03-0808 and ID03-0809) formed a group with high relatedness (98-100 %) and showed low relatedness to the other strains studied. The two strains in group III (ID03-0810(T) and ID03-0813) showed 58-68 % relatedness to Actinokineospora terrae NBRC 15668(T) and showed low relatedness (2-24 %) to the other strains studied. The description of three novel species is proposed: Actinokineospora baliensis sp. nov., for the single strain in group I (type strain ID03-0561(T) =BTCC B-554(T) =NBRC 104211(T)), Actinokineospora cibodasensis sp. nov., for the strains in group II (type strain ID03-0784(T) =BTCC B-555(T) =NBRC 104212(T)), and Actinokineospora cianjurensis sp. nov., for the strains in group III (type strain ID03-0810(T) =BTCC B-558(T) =NBRC 105526(T)).
从印度尼西亚土壤和植物凋落物样本中分离出的 6 株放线菌菌株，通过多相研究方法研究其分类地位。系统发育分析表明，所有菌株均位于游动放线菌属的宽簇中。化学分类学数据[细胞壁二氨基庚二酸、meso-二氨基庚二酸；细胞壁肽聚糖，III 型（A1γ）；主要糖，半乳糖和阿拉伯糖；主要类异戊二烯醌，MK-9（H₄）；主要脂肪酸，iso-C₁₆:₀；主要磷脂，磷脂酰乙醇胺]支持所有 6 株菌均属于游动放线菌属。与具有有效发表名称的游动放线菌属种的 DNA 进行 DNA-DNA 杂交的结果表明存在 3 个 DNA-DNA 相关群。第 I 组（ID03-0561(T)）与所研究的其他菌株的相关性较低。第 II 组（ID03-0784(T)、ID03-0808 和 ID03-0809）的 3 个菌株形成一个高度相关的组（98-100%），与所研究的其他菌株相关性较低。第 III 组（ID03-0810(T)和 ID03-0813）的 2 个菌株与游动放线菌属 terrae NBRC 15668(T)的相关性为 58-68%，与所研究的其他菌株的相关性较低（2-24%）。提出了三个新种的描述：巴厘游动放线菌 sp. nov.，用于第 I 组（单一菌株 ID03-0561(T) =BTCC B-554(T) =NBRC 104211(T)），西多沙游动放线菌 sp. nov.，用于第 II 组（菌株 ID03-0784(T) =BTCC B-555(T) =NBRC 104212(T)），以及詹吉尔游动放线菌 sp. nov.，用于第 III 组（菌株 ID03-0810(T) =BTCC B-558(T) =NBRC 105526(T)）。
