Wei Jie-Chao, Sun Le-Ni, Yuan Zhuo-Xin, Hou Xue-Ting, Yang En-Dong, Cao Yuan-Yuan
School of Life Science, Anhui Agricultural University, Hefei 230036, PR China.
Int J Syst Evol Microbiol. 2017 Aug;67(8):3099-3104. doi: 10.1099/ijsem.0.002101. Epub 2017 Aug 18.
A Gram-stain-negative, aerobic, non-motile, rod-shaped and non-spore-forming bacterium, designated EF23T, was isolated from rhizosphere soil of watermelon. Growth of strain EF23T was observed at 10-37 °C, at pH 5.0-9.0 and in the presence of 0-0.5 % (w/v) NaCl. Strain EF23T contained menaquinone 7 (MK-7) as the major isoprenoid quinone, and summed feature 3 (C16:1ω7c and/or iso-C15 : 0 2-OH), iso-C15 : 0, C16 : 0 and iso-C17 : 0 3-OH as the major fatty acids. Phosphatidylethanolamine was identified as the major polar lipid. The genomic DNA G+C content of strain EF23T was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain EF23T was most closely related to Mucilaginibacter gossypii Gh-67T (98.9 % similarity) and Mucilaginibacter gossypiicola Gh-48T (97.6 %). DNA-DNA relatedness values between strain EF23T and M. gossypii KCTC 22380T and M. gossypiicola KCTC 22379T were 31.6 and 53.7 %. On the basis of the evidence presented in this polyphasic taxonomic study, strain EF23T is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter rubeus sp. nov. is proposed. The type strain is EF23T (=CGMCC 1.15913T=KCTC 52516T).
从西瓜根际土壤中分离出一株革兰氏染色阴性、需氧、无运动性、杆状且不形成芽孢的细菌，命名为EF23T。在10-37°C、pH 5.0-9.0以及0-0.5%（w/v）NaCl存在的条件下观察到菌株EF23T的生长。菌株EF23T含有甲基萘醌7（MK-7）作为主要的类异戊二烯醌，主要脂肪酸为总和特征3（C16:1ω7c和/或异-C15:0 2-OH）、异-C15:0、C16:0和异-C17:0 3-OH。磷脂酰乙醇胺被鉴定为主要的极性脂质。菌株EF23T的基因组DNA G+C含量为43.7 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株EF23T与棉黏液杆菌Gh-67T（相似度98.9%）和棉黏液菌Gh-48T（相似度97.6%）关系最为密切。菌株EF23T与棉黏液杆菌KCTC 22380T和棉黏液菌KCTC 22379T之间的DNA-DNA相关性值分别为31.6%和53.7%。基于这一多相分类研究提供的证据，菌株EF23T被认为代表黏液杆菌属的一个新物种，为此提出名为黏液杆菌红球菌新种（Mucilaginibacter rubeus sp. nov.）。模式菌株为EF23T（=CGMCC 1.15913T=KCTC 52516T）。
