SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.
College of Marine Science, Shandong University, Weihai 264209, PR China.
Int J Syst Evol Microbiol. 2019 Dec;69(12):3885-3890. doi: 10.1099/ijsem.0.003702.
A Gram-stain-negative, aerobic, non-motile, rod-shaped, cold-tolerant bacterium, designated F01003, was isolated from soil sampled near Happiness Bay on the west coast of Antarctica. Strain F01003 was found to grow at 4-30 °C (optimum, 25 °C), pH 5.5-8.0 (pH 6.5-7.0) and in the presence of 0-1 % NaCl (0 %, w/v). Cells were oxidase-positive and catalase-positive. Strain F01003 contained menaquinone 7 (MK-7) as the predominant respiratory quinone. The main cellular fatty acids included summed feature 3 (Cω6 and/or Cω7) and iso-C. Phosphatidylethanolamine and an unidentified aminolipid were identified as the major polar lipids. The DNA G+C content of strain F01003 was 44.8 mol%. Phylogenetic analysis of the nearly full-length 16S rRNA gene sequence revealed that strain F01003 was most closely related to the genus and exhibited the highest sequence similarity to LMG 29118 (97.3 %). On the basis of the evidence presented in this polyphasic taxonomic study, strain F01003 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is F01003 (=KCTC 62991=CCTCC AB 2019023).
一株革兰氏阴性、需氧、不运动、杆状、耐冷的细菌，被命名为 F01003，从南极洲西海岸幸福湾附近的土壤中分离得到。菌株 F01003 被发现能在 4-30°C（最适温度 25°C）、pH5.5-8.0（pH6.5-7.0）和 0-1%NaCl（0%，w/v）的条件下生长。细胞呈氧化酶阳性和过氧化氢酶阳性。菌株 F01003 含有menaquinone 7（MK-7）作为主要的呼吸醌。主要的细胞脂肪酸包括总和特征 3（Cω6 和/或 Cω7）和 iso-C。鉴定出磷脂酰乙醇胺和一种未鉴定的胺脂为主要极性脂。菌株 F01003 的 DNA G+C 含量为 44.8mol%。近全长 16S rRNA 基因序列的系统发育分析表明，菌株 F01003 与属最为密切相关，与 LMG 29118（97.3%）的序列相似性最高。基于本多相分类学研究中提供的证据，菌株 F01003 被认为代表了属的一个新种，建议将其命名为 sp. nov.。模式菌株为 F01003（=KCTC 62991=CCTCC AB 2019023）。
