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sp. nov.,分离自南极土壤。

sp. nov., isolated from Antarctic soil.

机构信息

SOA Key Laboratory for Polar Science, Polar Research Institute of China, Shanghai 200136, PR China.

College of Marine Science, Shandong University, Weihai 264209, PR China.

出版信息

Int J Syst Evol Microbiol. 2019 Dec;69(12):3885-3890. doi: 10.1099/ijsem.0.003702.

Abstract

A Gram-stain-negative, aerobic, non-motile, rod-shaped, cold-tolerant bacterium, designated F01003, was isolated from soil sampled near Happiness Bay on the west coast of Antarctica. Strain F01003 was found to grow at 4-30 °C (optimum, 25 °C), pH 5.5-8.0 (pH 6.5-7.0) and in the presence of 0-1 % NaCl (0 %, w/v). Cells were oxidase-positive and catalase-positive. Strain F01003 contained menaquinone 7 (MK-7) as the predominant respiratory quinone. The main cellular fatty acids included summed feature 3 (Cω6 and/or Cω7) and iso-C. Phosphatidylethanolamine and an unidentified aminolipid were identified as the major polar lipids. The DNA G+C content of strain F01003 was 44.8 mol%. Phylogenetic analysis of the nearly full-length 16S rRNA gene sequence revealed that strain F01003 was most closely related to the genus and exhibited the highest sequence similarity to LMG 29118 (97.3 %). On the basis of the evidence presented in this polyphasic taxonomic study, strain F01003 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is F01003 (=KCTC 62991=CCTCC AB 2019023).

摘要

一株革兰氏阴性、需氧、不运动、杆状、耐冷的细菌,被命名为 F01003,从南极洲西海岸幸福湾附近的土壤中分离得到。菌株 F01003 被发现能在 4-30°C(最适温度 25°C)、pH5.5-8.0(pH6.5-7.0)和 0-1%NaCl(0%,w/v)的条件下生长。细胞呈氧化酶阳性和过氧化氢酶阳性。菌株 F01003 含有menaquinone 7(MK-7)作为主要的呼吸醌。主要的细胞脂肪酸包括总和特征 3(Cω6 和/或 Cω7)和 iso-C。鉴定出磷脂酰乙醇胺和一种未鉴定的胺脂为主要极性脂。菌株 F01003 的 DNA G+C 含量为 44.8mol%。近全长 16S rRNA 基因序列的系统发育分析表明,菌株 F01003 与属最为密切相关,与 LMG 29118(97.3%)的序列相似性最高。基于本多相分类学研究中提供的证据,菌株 F01003 被认为代表了属的一个新种,建议将其命名为 sp. nov.。模式菌株为 F01003(=KCTC 62991=CCTCC AB 2019023)。

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