Semyung University, 65, Semyung -ro, Jecheon-si, Chungcheongbuk-do 27136, Republic of Korea.
Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeongeup-si, Jeollabuk-do 56212, Republic of Korea.
Int J Syst Evol Microbiol. 2021 Feb;71(2). doi: 10.1099/ijsem.0.004617.
A novel Gram-negative bacterium, designated G2-14, was isolated from rhizosphere soil sample collected from apple orchard in Chungju-si, Chungcheongbuk-do, Republic of Korea. Strain G2-14 was a strictly aerobic, non-spore-forming, non-motile and short-rod-shaped bacterium. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain G2-14 was closely related to HMD1056 (96.9 %) and BDR-9 (96.8 %). The major cellular fatty acids (>10 %) of strain G2-14 were summed feature 3 (C 6 and/or C 7) and iso-C. The predominant quinone and the major polar lipid were menaquinone-7 and phosphatidylethanolamine, respectively. Strain G2-14 produced acetic acid. The DNA G+C content based on whole genome sequences was 46.4 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain G2-14 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is G2-14 (=KCTC 72533=NBRC 114179).
一株新的革兰氏阴性菌，命名为 G2-14，从韩国忠清北道忠州市苹果园的根际土壤样本中分离得到。菌株 G2-14 是一种严格需氧、非孢子形成、非运动和短杆状的细菌。基于 16S rRNA 基因序列的系统发育分析表明，菌株 G2-14 与 HMD1056（96.9%）和 BDR-9（96.8%）密切相关。菌株 G2-14 的主要细胞脂肪酸（>10%）为总和特征 3（C6 和/或 C7）和异-C。主要的醌类和主要的极性脂类分别是甲萘醌-7 和磷脂酰乙醇胺。菌株 G2-14 产生乙酸。基于全基因组序列的 DNA G+C 含量为 46.4 mol%。基于表型、化学分类和系统发育数据，菌株 G2-14 代表了属中的一个新种，因此建议使用新种名 。模式菌株为 G2-14（=KCTC 72533=NBRC 114179）。
