a Department of Biochemistry and Molecular Biology , Graduate School and Faculty of Medicine, The University of Tokyo , Tokyo , Japan.
b Division of Cancer Biology , National Cancer Center Research Institute , Tokyo , Japan.
Autophagy. 2017 Oct 3;13(10):1619-1628. doi: 10.1080/15548627.2017.1343770. Epub 2017 Aug 18.
Discovery of yeast autophagy-related (ATG) genes and subsequent identification of their homologs in other organisms have enabled researchers to investigate physiological functions of macroautophagy/autophagy using genetic techniques. Specific identification of autophagy-related structures is important to evaluate autophagic activity, and specific ablation of autophagy-related genes is a critical means to determine the requirements of autophagy. Here, we review currently available mouse models, particularly focusing on autophagy (and mitophagy) indicator models and systemic autophagy-related gene-knockout mouse models.
酵母自噬相关（ATG）基因的发现，以及随后在其他生物体中鉴定其同源物，使研究人员能够利用遗传技术研究巨自噬/自噬的生理功能。自噬相关结构的特异性鉴定对于评估自噬活性很重要，而自噬相关基因的特异性缺失是确定自噬需求的关键手段。在这里，我们综述了现有的小鼠模型，特别是侧重于自噬（和线粒体自噬）指示模型和系统性自噬相关基因敲除小鼠模型。
