Amkul Kitiya, Laosatit Kularb, Somta Prakit, Shim Sangrea, Lee Suk-Ha, Tanya Patcharin, Srinives Peerasak
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand.
Department of Plant Science and Research Institute of Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul 151-921, Korea.
Genes (Basel). 2017 Aug 18;8(8):205. doi: 10.3390/genes8080205.
Jatropha ( L.) is an oil-bearing plant that has potential to be cultivated as a biodiesel crop. The seed cake after oil extraction has 40-50% protein that can be used in animal feeds. A major limitation in utilizing the cake is the presence of phorbol esters (PE), a heat-tolerant toxic chemical. To identify the quantitative trait loci (QTLs) for PE, we constructed a genetic linkage map from an F₂ population of 95 individuals from a cross "Chai Nat" × "M10" using 143 simple sequence repeat (SSR) markers. M10 is low in seed PE while Chai Nat is high. Seeds from each F₂ individual were quantified for PE content by high performance liquid chromatography. A single marker analysis revealed five markers from linkage group 3 (LG3) and nine markers from LG8 associated with seed PE. Inclusive composite interval mapping identified two QTLs, each on LG3 () and LG8 () responsible for the PE. and accounted for 14.10%, and 15.49% of total variation in seed PE, respectively. Alelle(s) from M10 at increased seed PE, while at decreased seed PE. is a new loci for PE, while is the same locus with that reported recently for PE.
麻风树属植物是一种含油植物，有潜力作为生物柴油作物进行种植。榨油后的籽饼含有40%-50%的蛋白质，可用于动物饲料。利用该籽饼的一个主要限制因素是存在佛波酯（PE），这是一种耐热的有毒化学物质。为了鉴定PE的数量性状位点（QTL），我们使用143个简单序列重复（SSR）标记，从“猜纳”דM10”杂交产生的95个个体的F₂群体构建了遗传连锁图谱。M10种子中的PE含量低，而猜纳的含量高。通过高效液相色谱法对每个F₂个体的种子进行PE含量定量分析。单标记分析揭示了来自连锁群3（LG3）的5个标记和来自LG8的9个标记与种子PE相关。包含复合区间作图法鉴定出两个QTL，分别位于LG3（）和LG8（）上，与PE有关。和分别占种子PE总变异的14.10%和15.49%。M10在处的等位基因增加了种子PE含量，而在处则降低了种子PE含量。是PE的一个新位点，而与最近报道的PE位点相同。
