Department of Radiology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China (mainland).
Department of Radiology, The People's Hospital of China Medical University and The People's Hospital of Liaoning Province, Shenyang, Liaoning, China (mainland).
Med Sci Monit. 2017 Aug 18;23:3996-4003. doi: 10.12659/msm.902986.
BACKGROUND The aim of this study was to evaluate the application of diffusion kurtosis imaging (DKI) in spinal cord ischemia/reperfusion (SCI/R) injury and to explore its association with pathology. MATERIAL AND METHODS Japanese male long-eared rabbits were chosen and divided into 7 groups (8 rabbits in each group): control group (C group), sham-operation control group (S group), and 5 experimental groups (E-2 h group, E-24 h group, E-48 h group, E-7 d group, and E-14 d group). Tarlov scoring and immunohistochemical staining were used to assess hindlimb motor function and observe the expression of glial fiber acidic protein (GFAP), respectively. The correlation between DKI and pathology after SCI/R injury was compared by 3.0TMR scanning DKI. RESULTS Neuroethology in each time point of E groups was significantly different from that in C and S groups (P<0.05). The E-24 h group had the lowest value (P<0.05), and the hindlimb motor function began to recover after 24 h. The expression of GFAP was gradually increased after SCI/R injury, and the maximum value was in the E-7 d group (P<0.05). MK (mean kurtosis) had a linear negative correlation with average optical density (OD) (r=-0.115, P<0.05) and was positively correlated with integral OD (IOD) (r=0.204, P<0.05), in which MD (mean dispersion) was positively correlated with OD and IOD, but without a significant difference (r=0.618, r=251, P>0.05). CONCLUSIONS DKI can be used to monitor the changes in SCI/R injury, and fractional anisotropy (FA) can reflect change in white matter structure. The changes in expression of MK and GFAP were related to the myelin sheath injury repair process.
本研究旨在评估扩散峰度成像（DKI）在脊髓缺血再灌注（SCI/R）损伤中的应用，并探讨其与病理学的关系。
选择日本雄性大耳白兔，分为 7 组（每组 8 只）：对照组（C 组）、假手术对照组（S 组）和 5 个实验组（E-2 h 组、E-24 h 组、E-48 h 组、E-7 d 组和 E-14 d 组）。采用 Tarlov 评分和免疫组织化学染色分别评估后肢运动功能，观察神经胶质纤维酸性蛋白（GFAP）的表达。通过 3.0TMR 扫描 DKI 比较 SCI/R 损伤后 DKI 与病理学的相关性。
E 组各时间点的神经行为学与 C 组和 S 组比较差异均有统计学意义（P<0.05）。E-24 h 组评分最低（P<0.05），24 h 后后肢运动功能开始恢复。SCI/R 损伤后 GFAP 表达逐渐增加，E-7 d 组表达最高（P<0.05）。MK（平均峰度）与平均光密度（OD）呈线性负相关（r=-0.115，P<0.05），与积分 OD（IOD）呈正相关（r=0.204，P<0.05），其中 MD（平均弥散度）与 OD 和 IOD 呈正相关，但差异无统计学意义（r=0.618，r=251，P>0.05）。
DKI 可用于监测 SCI/R 损伤的变化，FA 可反映白质结构的变化。MK 和 GFAP 表达的变化与髓鞘损伤修复过程有关。
