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从食物中毒暴发中分离出的金黄色葡萄球菌中葡萄球菌肠毒素基因的流行情况与表达

Prevalence and expression of staphylococcal enterotoxin genes in Staphylococcus aureus isolated from food poisoning outbreaks.

作者信息

Bastos Caroline Peixoto, Bassani Milena Tomasi, Mata Marcia Magalhães, Lopes Graciela Volz, da Silva Wladimir Padilha

机构信息

Federal University of Pelotas (UFPel), Faculty of Agronomy Eliseu Maciel (FAEM), Department of Science and Food Technology, Pelotas, RS, Brazil.

出版信息

Can J Microbiol. 2017 Oct;63(10):834-840. doi: 10.1139/cjm-2017-0316. Epub 2017 Aug 18.

DOI:10.1139/cjm-2017-0316
PMID:28820948
Abstract

Staphylococcus aureus is an important pathogen of foodborne origin. The pathogen produces a variety of toxins that include the staphylococcal enterotoxins (SE). The present study aimed to evaluate the prevalence and expression of 5 SE genes (sea, seb, sec, sed, and see) in S. aureus isolated from outbreaks occurred in the state of Rio Grande do Sul, Brazil. All isolates, with the exception of 2, presented the same or higher transcriptional expression than the reference strains for at least 1 of these genes. The presence of SE genes combined with high levels of transcriptional expression suggests that 1 or more SEs were involved with the staphylococcal food poisoning outbreak analyzed in the present study.

摘要

金黄色葡萄球菌是一种重要的食源性病原体。该病原体产生多种毒素,包括葡萄球菌肠毒素(SE)。本研究旨在评估从巴西南里奥格兰德州发生的疫情中分离出的金黄色葡萄球菌中5种SE基因(sea、seb、sec、sed和see)的流行情况和表达情况。除2株外,所有分离株至少有1个基因的转录表达与参考菌株相同或更高。SE基因的存在与高水平的转录表达表明,在本研究分析的葡萄球菌食物中毒疫情中,有1种或更多种SE参与其中。

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