College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Beijing JTM International Food Co., Ltd., Beijing 101400, China.
Toxins (Basel). 2022 Oct 21;14(10):718. doi: 10.3390/toxins14100718.
Pathogens and mycotoxins are serious public health risks for humans and food safety in milk. This study concentrated on detecting and Ochratoxin A (OTA) in 210 pasteurized milk from ten urban Beijing districts to suggest the co-occurrence of with toxin-producing genes and OTA in milk and the possible risk. was identified by physiological and biochemical experiments and molecular biology experiments, and enterotoxin genes were identified by PCR. OTA was detected by LC-MS/MS. The study found 29 isolates of , of which 17.24% had the sea gene encoding enterotoxin A. OTA was detected in 31 out of 120 samples and the maximum amount of detection was 18.8 μg/kg. The results of this study indicate that when failing to guarantee the cold chain, the presence of with enterotoxin genes in milk will present a risk to food safety. Furthermore, the high detection rates and levels of OTA in milk suggest that OTA is a hidden risk. The co-occurrence of and OTA in milk is a food safety concern and there is a need to control the occurrence of these two biohazards in milk.
病原体和真菌毒素是人类健康和食品安全的严重威胁。本研究集中检测了北京十个城区 210 份巴氏杀菌乳中的 ,并探讨了产毒基因与真菌毒素 Ochratoxin A(OTA)在奶中的共同存在及其潜在风险。通过生理生化实验和分子生物学实验鉴定出 ,并通过 PCR 鉴定出肠毒素基因。采用 LC-MS/MS 检测 OTA。研究共检出 29 株 ,其中 17.24%具有编码肠毒素 A 的 sea 基因。在 120 个样本中有 31 个检出 OTA,检出量最高为 18.8μg/kg。本研究表明,在冷链无法保证的情况下,奶中存在产毒基因的 可能会对食品安全构成威胁。此外,奶中 OTA 的高检出率和高检出水平表明 OTA 是一个隐藏的风险。奶中 与 OTA 的共同存在是食品安全关注的问题,需要控制这两种生物危害在奶中的发生。