Nicodemou Andreas, Danisovic Lubos
Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Sasinkova 4, 811 08, Bratislava, Slovakia.
Regenmed Ltd., Medená 29, 811 08, Bratislava, Slovakia.
Cell Tissue Bank. 2017 Dec;18(4):443-460. doi: 10.1007/s10561-017-9658-x. Epub 2017 Aug 18.
Mesenchymal stem (stromal) cells (MSCs) possess unique biological characteristics such as plasticity, long term self-renewal, secretion of various bioactive molecules and ability of active migration to the diseased tissues that make them unique tool for regenerative medicine, nowadays. Until now MSCs were successfully derived from many tissue sources including bone marrow, umbilical cord, adipose tissue, dental pulp etc. The crucial step prior to their in vitro expansion, banking or potential clinical application is their separation. This review article aims to briefly describe the main MSCs separations techniques currently available, their basic principles, as well as their advantages and limits. In addition the attention is paid to the markers presently applicable for immunoaffinity-based separation of MSCs from different tissues and organs.
间充质干（基质）细胞（MSCs）具有独特的生物学特性，如可塑性、长期自我更新、分泌各种生物活性分子以及向患病组织主动迁移的能力，这些特性使其成为当今再生医学的独特工具。到目前为止，MSCs已成功从许多组织来源获得，包括骨髓、脐带、脂肪组织、牙髓等。在其体外扩增、储存或潜在临床应用之前的关键步骤是它们的分离。这篇综述文章旨在简要描述目前可用的主要MSCs分离技术、其基本原理以及它们的优点和局限性。此外，还关注目前适用于基于免疫亲和从不同组织和器官中分离MSCs的标志物。
