State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
China Northwest Architecture Design and Research Institute Co. Ltd., Xi'an 710018, China.
Int J Environ Res Public Health. 2017 Nov 30;14(12):1483. doi: 10.3390/ijerph14121483.
High solid anaerobic digestion (HSAD) is a rapidly developed anaerobic digestion technique for treating municipal sludge, and has been widely used in Europe and Asia. Recently, the enhanced HSAD process with thermal treatment showed its advantages in both methane production and VS reduction. However, the understanding of the microbial community is still poor. This study investigated microbial communities in a pilot enhanced two-stage HSAD system that degraded waste activated sludge at 9% solid content. The system employed process "thermal pre-treatment (TPT) at 70 °C, thermophilic anaerobic digestion (TAD), and mesophilic anaerobic digestion (MAD)". Hydrogenotrophic methanogens spp. dominated the system with relative abundance up to about 100% in both TAD and MAD. Syntrophic acetate oxidation (SAO) bacteria were discovered in TAD, and they converted acetate into H₂ and CO₂ to support hydrogenotrophic methanogenesis. The microbial composition and conversion route of this system are derived from the high solid content and protein content in raw sludge, as well as the operational conditions. This study could facilitate the understanding of the enhanced HSAD process, and is of academic and industrial importance.
高固体厌氧消化（HSAD）是一种快速发展的用于处理城市污泥的厌氧消化技术，已在欧洲和亚洲得到广泛应用。最近，具有热处理的增强型 HSAD 工艺在甲烷生产和 VS 减少方面显示出优势。然而，对微生物群落的理解仍然很差。本研究调查了在一个中试增强型两阶段 HSAD 系统中的微生物群落，该系统在 9%固体含量下降解废活性污泥。该系统采用了“70°C 热预处理（TPT）、嗜热厌氧消化（TAD）和中温厌氧消化（MAD）”工艺。产氢甲烷菌属占主导地位，在 TAD 和 MAD 中相对丰度高达约 100%。在 TAD 中发现了乙酸盐氧化菌（SAO），它们将乙酸盐转化为 H₂和 CO₂，以支持产氢甲烷生成。该系统的微生物组成和转化途径源自原料污泥中的高固体含量和蛋白质含量，以及操作条件。本研究有助于理解增强型 HSAD 工艺，具有学术和工业重要性。
