Biotechnology and Synthetic Biology, Center of Innovative and Applied Bioprocessing, Knowledge City, Sector-81, Mohali 140306, India.
Biotechnology and Synthetic Biology, Center of Innovative and Applied Bioprocessing, Knowledge City, Sector-81, Mohali 140306, India.
Bioresour Technol. 2017 Nov;244(Pt 1):793-799. doi: 10.1016/j.biortech.2017.08.034. Epub 2017 Aug 9.
Most of the chemical and biochemical processes used for the de-polymerization of structural polymers of lignocellulosic biomass are environment unfriendly and costly. Here an efficient process based on xylanase, produced by Acinetobacter pittii MASK25 (MTCC 25132), hydrolysis of only physically treated rice straw and corn cob has been developed for the production of xylooligosaccharides. Bacterial strain isolated from soil was found to produce maximum xylanase at 30°C and pH 7. While the optimum temperature and pH of xylanase were characterized as 40°C and 5. Process was further improved by developing magnetic-xylanase CLEA. Crude xylanase and magnetic-xylanase CLEA could convert respectively more than 45% and 60% xylan of the powdered rice straw and corn cob into xylooligosaccharides. Interestingly, hydrolysis by both types of enzymatic forms was found to produce predominantly xylopentose and xylohexose. Hence, the process is environment friendly and the predominant production of xylopentose and xylohexose could find unique prebiotic applications.
大多数用于木质纤维素生物质结构聚合物解聚的化学和生化过程都对环境不友好且成本高昂。在这里，我们开发了一种基于木聚糖酶的高效工艺，该木聚糖酶由不动杆菌属（Acinetobacter pittii）MASK25（MTCC 25132）产生，仅对物理处理过的稻草和玉米芯进行水解，即可生产木二糖寡糖。从土壤中分离出的菌株被发现可在 30°C 和 pH 值 7 的条件下产生最大量的木聚糖酶。该木聚糖酶的最适温度和 pH 值分别为 40°C 和 5。通过开发磁性木聚糖酶 CLEA，进一步改进了该工艺。粗木聚糖酶和磁性木聚糖酶 CLEA 分别可以将粉末状稻草和玉米芯中的 45%以上和 60%以上的木聚糖转化为木二糖寡糖。有趣的是，两种酶形式的水解都被发现主要产生木戊糖和木己糖。因此，该工艺具有环境友好性，并且主要生产木戊糖和木己糖可能会找到独特的益生元应用。
