Swedish Centre for Resource Recovery, University of Borås, SE-50190 Borås, Sweden.
Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman 7618868366, Iran.
Molecules. 2019 Feb 17;24(4):721. doi: 10.3390/molecules24040721.
Integrated enzyme production in the biorefinery can significantly reduce the cost of the entire process. The purpose of the present study is to evaluate the production of two hydrolyzing enzymes (amylase and xylanase) by an edible fungus used in the biorefinery, . The enzyme production was explored through submerged fermentation of synthetic media and a wheat-based waste stream (thin stillage and wheat bran). The influence of a nitrogen source on was investigated and a combination of NaNO₃ and yeast extract has been identified as the best nitrogen source for extracellular enzyme production. enzymes showed maximum activity at 65 °C and pH around 5. Under these conditions, the maximum velocity of amylase and xylanase for starch and xylan hydrolysis was found to be 3.25 U mL and 14.77 U mL, respectively. Cultivation of in thin stillage and wheat bran medium resulted in relatively high amylase (8.86 ± 0.41 U mL, 4.68 ± 0.23) and xylanase (5.48 ± 0.21, 2.58 ± 0.07 U mL) production, respectively, which makes this fungus promising for enzyme production through a wheat-based biorefinery.
生物炼制中集成酶生产可以显著降低整个过程的成本。本研究的目的是评估在生物炼制中使用的一种食用真菌产生两种水解酶（淀粉酶和木聚糖酶）的情况。通过合成培养基和小麦基废物流（稀酒糟和麦麸）的深层发酵探索了酶的生产。研究了氮源对 的影响，发现 NaNO₃和酵母提取物的组合是产细胞外酶的最佳氮源。 酶在 65°C 和 pH 约 5 时表现出最大活性。在此条件下，淀粉和木聚糖水解的淀粉酶和木聚糖酶的最大速度分别为 3.25 U mL 和 14.77 U mL。在稀酒糟和麦麸培养基中培养 导致相对较高的淀粉酶（8.86 ± 0.41 U mL，4.68 ± 0.23）和木聚糖酶（5.48 ± 0.21，2.58 ± 0.07 U mL）的产生，这使得这种真菌有望通过基于小麦的生物炼制进行酶生产。
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