Chen Weinan, Zhu Bowen, Ma Li, Hua Xiaoqing
School of Mechatronic Engineering and Automation, 34747 Shanghai University , Shanghai, China.
J Biomater Appl. 2017 Oct;32(4):504-510. doi: 10.1177/0885328217726439. Epub 2017 Aug 21.
In this study, we propose an electrodeposition method of fabricating shape-controlled calcium alginate-poly-L-lysine hydrogel microcapsules. The micro-patterned electrodes, which are produced by coating a patterned photoresist layer onto fluorine-doped tin oxide glass slide based on photolithography technique, are used for making different shapes of microcapsules. By the electrolysis of water in alginate gelation on micro-patterned anode electrode, the 2D alginate hydrogel structures embedded with yeast cells are formed on fluorine-doped tin oxide glass slide. Then, the 2D structures would be detached from the microelectrode surface and treated with given reagent to be transformed into 3D microcapsules while maintaining the ring and hexagon shapes. Finally, the yeast cells within the microcapsules are further promoted into compact tissues by cultivation. The experimental results indicate the method can successfully fabricate tissues which can maintain certain cells bioactivity after 24 h cultivation. The recommended method can lead to fabricating cell-laden scaffold for tissue engineering, biological studies and drug discovery with higher accuracy and efficiency.
在本研究中，我们提出了一种制备形状可控的海藻酸钙-聚-L-赖氨酸水凝胶微胶囊的电沉积方法。通过基于光刻技术在氟掺杂氧化锡玻璃载玻片上涂覆图案化光刻胶层制备的微图案电极，用于制备不同形状的微胶囊。通过在微图案化阳极电极上对海藻酸盐凝胶化过程中的水进行电解，在氟掺杂氧化锡玻璃载玻片上形成嵌入酵母细胞的二维海藻酸盐水凝胶结构。然后，将二维结构从微电极表面分离，并用给定试剂处理，转化为三维微胶囊，同时保持环形和六边形形状。最后，通过培养进一步促进微胶囊内的酵母细胞形成致密组织。实验结果表明，该方法能够成功制备出在培养24小时后仍能保持一定细胞生物活性的组织。所推荐的方法能够以更高的精度和效率制备用于组织工程、生物学研究和药物发现的载细胞支架。
