Sawhney A S, Hubbell J A
Department of Chemical Engineering, University of Texas, Austin 78712-1062.
Biomaterials. 1992;13(12):863-70. doi: 10.1016/0142-9612(92)90180-v.
A graft copolymer having poly(L-lysine) (PLL) as the backbone and monomethoxy poly(ethylene glycol) (MPEG) as pendent chains was synthesized. This polycationic copolymer was used to form microcapsules with sodium alginate, a polyanion. Microcapsules and model surfaces formed with PLL-graft-MPEG demonstrated reduced protein adsorption, complement binding and cell adhesion in vitro compared to materials with unmodified PLL. Microcapsules with PLL-g-MPEG on the surface were seen to be much more biocompatible than the widely used alginate/PLL/alginate microcapsule in a mouse intraperitoneal implant model. The graft copolymers demonstrated a lower affinity for alginate and increased microcapsule permeability more than PLL. To correct this, pentalayered alginate/PLL/alginate/PLL-g-MPEG/alginate microcapsules were fabricated, and these demonstrated both appropriate permselectivity and enhanced biocompatibility.
合成了一种以聚（L-赖氨酸）（PLL）为主链、单甲氧基聚（乙二醇）（MPEG）为侧链的接枝共聚物。这种聚阳离子共聚物用于与聚阴离子海藻酸钠形成微胶囊。与未修饰的PLL材料相比，由PLL-接枝-MPEG形成的微胶囊和模型表面在体外表现出降低的蛋白质吸附、补体结合和细胞粘附。在小鼠腹腔植入模型中，表面带有PLL-g-MPEG的微胶囊比广泛使用的海藻酸盐/PLL/海藻酸盐微胶囊具有更高的生物相容性。与PLL相比，接枝共聚物对海藻酸盐的亲和力较低，微胶囊通透性增加。为了纠正这一点，制备了五层海藻酸盐/PLL/海藻酸盐/PLL-g-MPEG/海藻酸盐微胶囊，这些微胶囊表现出适当的渗透选择性和增强的生物相容性。
