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百岁老人和类风湿关节炎患者的 microRNA 表达分析显示出共同的表达模式。

MicroRNA Expression Analysis of Centenarians and Rheumatoid Arthritis Patients Reveals a Common Expression Pattern.

机构信息

Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy.

Associazione "L'Isola dei Centenari", Via Milano 4, Sassari 07100, Italy.

出版信息

Int J Med Sci. 2017 Jun 14;14(7):622-628. doi: 10.7150/ijms.18972. eCollection 2017.

DOI:10.7150/ijms.18972
PMID:28824293
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5562112/
Abstract

Micro-RNA (miRNA) are a family of small non-coding ribonucleic acids that inhibits post-transcriptionally the expression of their target messenger RNA (mRNA). We are interested in studying the involvement of miRNA in longevity and autoimmune diseases. In this study we compared the different expression of seven microRNAs between human plasma healthy controls, plasma samples of centenarians and samples from patients with rheumatoid arthritis. We used the Life Technologies' protocol to quantify seven miRNAs from 62 plasma samples: 20 healthy human controls, 14 centenarians, 28 patients with rheumatoid arthritis. TaqMan MicroRNA assays were used to analyze the expression profiles of miR-125b-5p, miR-425-5p, miR-200b5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-21-5p and miR-126-3p. The relative expression of mature miRNAs was analyzed using software REST. Our results show that miR-425-5p, miR-21 and miR-212 significantly decreased in centenarians and in patients with rheumatoid arthritis compared with controls. Furthermore in this work we highlight a connection between corticosteroid treatment and miRNAs expression.

摘要

微 RNA (miRNA) 是一类小的非编码核糖核酸，通过转录后抑制其靶信使 RNA (mRNA) 的表达。我们有兴趣研究 miRNA 在长寿和自身免疫性疾病中的作用。在这项研究中，我们比较了人血浆健康对照组、百岁老人血浆样本和类风湿关节炎患者样本中七种 microRNA 的不同表达。我们使用 Life Technologies 的方案从 62 个血浆样本中定量七种 miRNA：20 个健康人类对照、14 个百岁老人、28 个类风湿关节炎患者。TaqMan microRNA 检测用于分析 miR-125b-5p、miR-425-5p、miR-200b5p、miR-200c-3p、miR-579-3p、miR-212-3p、miR-21-5p 和 miR-126-3p 的表达谱。成熟 miRNA 的相对表达使用软件 REST 进行分析。我们的结果表明，与对照组相比，miR-425-5p、miR-21 和 miR-212 在百岁老人和类风湿关节炎患者中显著降低。此外，在这项工作中，我们强调了皮质类固醇治疗与 miRNA 表达之间的联系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2e/5562112/a338e73139e0/ijmsv14p0622g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2e/5562112/ad45d4316895/ijmsv14p0622g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2e/5562112/a338e73139e0/ijmsv14p0622g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2e/5562112/ad45d4316895/ijmsv14p0622g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ea2e/5562112/a338e73139e0/ijmsv14p0622g002.jpg

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