Fang Zhongming, Bai Genxiang, Huang Weiting, Wang Zhixin, Wang Xuelu, Zhang Mingyong
Center of Applied Biotechnology, Wuhan Institute of BioengineeringWuhan, China.
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural UniversityWuhan, China.
Front Plant Sci. 2017 Aug 2;8:1338. doi: 10.3389/fpls.2017.01338. eCollection 2017.
Nitrogen use efficiency is important for the development of sustainable agriculture. Plants have different transporters to facilitate nitrogen uptake and internal distribution. This study demonstrates that the peptide transporter enhances nitrogen allocation and increases grain yield in rice. OsNPF7.3 is a member of the nitrate transporter 1/peptide transporter family (NPF) and is localized in the vacuolar membrane. Its expression is higher in the lateral roots and stems. Its transcripts concentrate in the vascular bundle and significantly regulated by organic nitrogen sources. The RNAi lines of affect plant growth and cause amino acids to accumulate in leaf sheaths and decrease in the leaf blades. At later stages of reproductive growth, nitrogen degradation accelerates in the leaves of plants over-expressing and the nitrogen is translocated to grains. The tiller numbers, panicles per plant, filled grain numbers per panicle, and grain nitrogen content of the over-expressing plant were more than that of wide type. The elevated gene expression in could enhance nitrogen utilization efficiency in rice paddy.
氮素利用效率对可持续农业发展至关重要。植物拥有不同的转运蛋白来促进氮素吸收和体内分配。本研究表明，肽转运蛋白可增强水稻中的氮素分配并提高籽粒产量。OsNPF7.3是硝酸盐转运蛋白1/肽转运蛋白家族（NPF）的成员，定位于液泡膜。其在侧根和茎中的表达较高。其转录本集中在维管束中，并受有机氮源的显著调控。OsNPF7.3的RNA干扰株系影响植物生长，导致氨基酸在叶鞘中积累而在叶片中减少。在生殖生长后期，过表达OsNPF7.3的植株叶片中的氮素降解加速，且氮素转运至籽粒中。过表达OsNPF7.3植株的分蘖数、单株穗数、每穗实粒数和籽粒氮含量均高于野生型。OsNPF7.3基因表达的提高可增强稻田中水稻的氮素利用效率。
