Serichantalergs Oralak, Ruekit Sirigade, Pandey Prativa, Anuras Sinn, Mason Carl, Bodhidatta Ladaporn, Swierczewski Brett
Department of Enteric Diseases, Armed Forces Research Institute of Medical Sciences, 315/6 Rajvithi Road, Bangkok, 10400 Thailand.
CIWEC Clinic Travel Medicine Center, GPO Box 12895, Kapurdhara Marg, Kathmandu, 44600 Nepal.
Gut Pathog. 2017 Aug 17;9:47. doi: 10.1186/s13099-017-0197-6. eCollection 2017.
and have emerged in recent years as being associated with acute and prolonged gastroenteritis and implicated in the development of inflammatory bowel diseases. However, there are limited data on the prevalence of these microorganisms in Southeast Asia. In this study, 214 pathogen-negative stool samples after laboratory examination for common enteric pathogens to include and by culture from two case-control traveler's diarrhea (TD) studies conducted in Thailand (cases = 26; controls = 30) and Nepal (cases = 83; controls = 75) respectively were assayed by PCR for the detection of 16S and two specific heat shock protein genes specific for () and () respectively.
16S was detected in 28.5% (61/214) of the pathogen-negative TD stool samples (CIWEC Travel Medicine Clinic, Kathmandu, Nepal: cases = 36, control = 14; Bamrungrad International Hospital, Bangkok, Thailand: cases = 9, controls = 2). was identified significantly more often in TD cases in Nepal (28.9%; 24/83) as compared to controls (4%; 3/75) (OR = 9.76; 95% CI 2.80-34.02; = 0.0003) while was detected in only two cases (2/26; 7.7%) and none of the controls stool samples from Thailand. was detected in four cases (4.8%; 4/83) and four controls (5.3%; 4/75) and in one case (3.8%; 1/26) and one control (3.1%; 1/30) from Nepal and Thailand respectively. and were isolated in 18.3 and 3.4% of the cases and in 4.0 and 1.4% of the controls in stool samples from both Thailand and Nepal respectively while nor were not tested for in these samples.
These findings suggest that potentially is a pathogen associated with TD in Nepal. To our knowledge, this is the first report of and detected from traveler's diarrhea cases from travelers to Nepal and Thailand.
近年来，[微生物名称1]和[微生物名称2]已被发现与急性和持续性肠胃炎有关，并与炎症性肠病的发展有关。然而，关于这些微生物在东南亚的流行情况的数据有限。在本研究中，对分别来自泰国（病例=26；对照=30）和尼泊尔（病例=83；对照=75）的两项病例对照旅行者腹泻（TD）研究中，经实验室检查排除常见肠道病原体（包括通过培养检测[相关病原体名称1]和[相关病原体名称2]）后的214份病原体阴性粪便样本，采用PCR法检测[微生物名称1]的16S核糖体RNA以及分别针对[微生物名称2]和[微生物名称3]的两个特异性热休克蛋白基因。
在214份病原体阴性的TD粪便样本中，28.5%（61/214）检测到16S核糖体RNA（尼泊尔加德满都CIWEC旅行医学诊所：病例=36，对照=14；泰国曼谷Bamrungrad国际医院：病例=9，对照=2）。与对照（4%；3/75）相比，尼泊尔TD病例中[微生物名称2]的检出率显著更高（28.9%；24/83）（比值比=9.76；95%置信区间2.80 - 34.02；P = 0.0003），而在泰国仅在2例（2/26；7.7%）病例中检测到[微生物名称3]，对照粪便样本中均未检测到。在尼泊尔的4例（4.8%；4/83）病例和4例（5.3%；4/75）对照中以及在泰国的1例（3.8%；1/26）病例和1例（3.1%；1/30）对照中检测到[微生物名称4]。在泰国和尼泊尔的粪便样本中，[微生物名称5]和[微生物名称6]分别在18.3%和3.4%的病例以及4.0%和1.4%的对照中分离得到，而在这些样本中未检测[微生物名称7]和[微生物名称8]。
这些发现表明，[微生物名称2]可能是尼泊尔TD的一种病原体。据我们所知，这是首次从前往尼泊尔和泰国的旅行者腹泻病例中检测到[微生物名称2]和[微生物名称3]的报告。
