Department of Oral Biology, The Goldschleger School of Dental Medicine, Sackler Faculty of Medicine, Tel Aviv University , Tel Aviv 69978, Israel.
Department of Materials Science and Engineering, Seoul National University , Seoul 151-744, Korea.
Biomacromolecules. 2017 Nov 13;18(11):3541-3550. doi: 10.1021/acs.biomac.7b00876. Epub 2017 Sep 12.
Hydrogels are promising candidates for biomimetic scaffolds of the extracellular matrix in tissue engineering applications. However, their use in bone tissue engineering is limited due to their low mechanical properties. In this study, we designed and synthesized multicomponent peptide-based hydrogels composed of fluorenyl-9-methoxycarbonyl diphenylalanine (FmocFF), which contributed to the rigidity and stability of the hydrogel, and Fmoc-arginine (FmocR), which mediated high affinity to hydroxyapatite (HAP) due to the arginine moiety. The new hydrogels composed of nanometric fibril networks were decorated with HAP and demonstrated high mechanical strength with a storage modulus of up to 29 kPa. In addition, the hydrogels supported cell adhesion and in vitro cell viability. These properties suggest using these multicomponent organic-inorganic hydrogels as functional biomaterials for improved bone regeneration.
水凝胶是组织工程应用中仿生细胞外基质支架的有前途的候选材料。然而，由于其机械性能低，其在骨组织工程中的应用受到限制。在这项研究中，我们设计并合成了由芴甲氧羰基二苯丙氨酸（FmocFF）组成的多组分肽基水凝胶，该水凝胶有助于水凝胶的刚性和稳定性，并且由于精氨酸部分，Fmoc-精氨酸（FmocR）介导对羟基磷灰石（HAP）的高亲和力。由纳米纤维网络组成的新型水凝胶用 HAP 修饰，并表现出高达 29 kPa 的高机械强度的存储模量。此外，水凝胶支持细胞黏附和体外细胞活力。这些特性表明，这些多组分有机-无机水凝胶可用作功能生物材料，以改善骨再生。
