Alves Lívia M, Rodovalho Vinícius R, Castro Ana C H, Freitas Márcia A R, Mota Caroline M, Mineo Tiago W P, Mineo José R, Madurro João M, Brito-Madurro Ana G
Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, Brazil.
Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia, Brazil.
J Pharm Biomed Anal. 2017 Oct 25;145:838-844. doi: 10.1016/j.jpba.2017.07.050. Epub 2017 Aug 9.
This work describes an approach for the selection and detection of specific DNA probes related to Toxoplasma gondii, a protozoan parasite responsible for toxoplasmosis. The detection system was developed on graphite carbon electrode modified with poly(3-hydroxybenzoic acid) sensitized with ToxG1 probe. The hybridization of the specific genomic DNA related to T. gondii showed good response by direct detection of guanine residue oxidation using differential pulse voltammetry (DPV). The biosensor was able to distinguish both the complementary and non-complementary targets and detect up to 100ngμL of the T. gondii genomic DNA. The hybridization (ToxG1: T. gondii genomic DNA) was confirmed by optical measurement. Optical assays using gold nanoparticles:ToxG1 probe showed a significant change in the absorbance peak in the presence of the T. gondii genomic DNA according to the electrochemical results. This novel biosensor shows potential as electrochemical transducer and was successfully applied in the biological sample.
这项工作描述了一种用于选择和检测与弓形虫相关的特定DNA探针的方法，弓形虫是一种导致弓形虫病的原生动物寄生虫。该检测系统是在涂有经ToxG1探针敏化的聚（3-羟基苯甲酸）的石墨碳电极上开发的。通过使用差分脉冲伏安法（DPV）直接检测鸟嘌呤残基氧化，与刚地弓形虫相关的特定基因组DNA的杂交显示出良好的响应。该生物传感器能够区分互补和非互补靶标，并能检测高达100ngμL的刚地弓形虫基因组DNA。通过光学测量证实了杂交（ToxG1：刚地弓形虫基因组DNA）。根据电化学结果，使用金纳米颗粒：ToxG1探针的光学测定显示，在刚地弓形虫基因组DNA存在下，吸光度峰值有显著变化。这种新型生物传感器显示出作为电化学传感器的潜力，并已成功应用于生物样品中。
