Pearson T W, Liu M, Gardiner I C, Longridge D, Beecroft R P, Sayer P D, Gould S S, Waitumbi J N, Njogu A R
Acta Trop. 1986 Dec;43(4):391-9.
Uncoated procyclic culture forms of African trypanosomes were used in immunofluorescence and simple agglutination assays to detect antibodies in the sera of vervet monkeys infected with T. b. rhodesiense. Antibodies to procyclic surface antigens were found in sera from animals with active, untreated infections or sera taken soon after treatment with trypanocidal drugs. The antibodies were detectable within 7 days of infection. No specific antibodies were detected in sera prior to infection or long after drug cure. The results indicate that antigens expressed on the surface of procyclic culture forms of T. brucei spp. are useful for the detection of antibodies produced in response to infection with T. b. rhodesiense and may allow the development of a simple immunodiagnostic test for African sleeping sickness. In addition, the use of a form of the trypanosome of a different differentiation state from the infecting organism illustrates the utility of this approach for detection of antibodies to common antigens.
非洲锥虫无包被的前循环期培养形式被用于免疫荧光和简单凝集试验，以检测感染罗德西亚布氏锥虫的绿猴血清中的抗体。在患有活动性、未经治疗感染的动物血清或在用杀锥虫药物治疗后不久采集的血清中，发现了针对前循环期表面抗原的抗体。感染后7天内即可检测到这些抗体。在感染前或药物治愈后很长时间的血清中未检测到特异性抗体。结果表明，布氏锥虫属前循环期培养形式表面表达的抗原可用于检测因感染罗德西亚布氏锥虫而产生的抗体，并可能有助于开发一种用于非洲昏睡病的简单免疫诊断测试。此外，使用与感染生物体处于不同分化状态的锥虫形式说明了这种方法在检测针对共同抗原的抗体方面的实用性。
