Ngaira J M, Olaho-Mukani W, Omuse J K, Tengekyon K M, Mbwabi D, Olado D, Njenga J N
Kenya Trypanosomiasis Research Institute, Muguaga, Kikuyu, Kenya.
Trop Med Parasitol. 1992 Mar;43(1):29-32.
Documented sera from 156 patients admitted to Alupe Sleeping Sickness Hospital in Western Kenya were tested to determine the potential usefulness of Procclic Agglutination Trypanosomiasis Test (PATT) for the diagnosis of Trypanosoma brucei rhodesiense African sleeping sickness. A total of 490 serum samples were tested, including 42 controls. Anti-trypanosome antibodies were detected in 99% of the sera taken prior to trypanocidal drug therapy. Antibody levels remained high during course of treatment. In cured cases antibodies declined to negative or low levels 4 months to one year after treatment. High antibody levels persisted in patients who relapsed. Although the results showed a high sensitivity and specificity, confirming the potential usefulness of the test for serodiagnosis of African sleeping sickness, PATT, in its present form is unsuitable for routine diagnosis. This is due to difficulties inherent in the use of live trypanosomes as detector antigen.
对肯尼亚西部阿鲁佩昏睡病医院收治的156例患者的血清样本进行检测，以确定循环凝集锥虫病试验（PATT）在诊断罗德西亚布氏锥虫引起的非洲昏睡病方面的潜在效用。共检测了490份血清样本，其中包括42份对照样本。在接受杀锥虫药物治疗前采集的血清样本中，99%检测到抗锥虫抗体。在治疗过程中抗体水平一直很高。在治愈的病例中，治疗后4个月至1年抗体水平降至阴性或低水平。复发患者的抗体水平持续较高。尽管结果显示该试验具有高敏感性和特异性，证实了其在非洲昏睡病血清诊断中的潜在效用，但目前形式的PATT不适合常规诊断。这是由于使用活锥虫作为检测抗原存在固有的困难。
