UNESCO- Trace Element Satellite Center School of Studies in Zoology, Jiwaji University, Gwalior, M.P., India.
UNESCO- Trace Element Satellite Center School of Studies in Zoology, Jiwaji University, Gwalior, M.P., India.
Regul Toxicol Pharmacol. 2017 Nov;90:36-41. doi: 10.1016/j.yrtph.2017.08.011. Epub 2017 Aug 19.
The present study was premeditated to demonstrate the hepatoprotective effect of silver nanoparticles (AgNPs). Rats were treated with three different doses of AgNPs (50, 100 and 150 μg/kg, p.o.) after Acetaminophen (APAP; 2 g/kg, p.o. once only) intoxication. Treatment with AgNPs recouped the levels of serum aspartate amino transaminase (AST), alanine amino transaminase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), bilirubin, triglyceride (TG) and cholesterol in dose dependent manner. Significant reduction in lipid peroxidation (LPO) and restoration of reduced glutathione (GSH) was found in liver in AgNPs treated animals. Alleviated activities of adenosine triphosphatase (ATPase), glucose-6- phosphatase (G6Pase) and antioxidant enzymes viz. superoxide dismutase (SOD) and catalase (CAT) due to APAP induced toxicity in liver were recovered by the treatment of AgNPs. Improvement in histoarchitecture of liver was also consistent with biochemical observations. The results revealed that AgNPs showed significant dose-dependent protection against APAP induced hepatocellular injury.
本研究旨在证明银纳米粒子（AgNPs）的肝保护作用。大鼠在单次口服对乙酰氨基酚（APAP；2 g/kg）后，用三种不同剂量的 AgNPs（50、100 和 150 μg/kg，po）进行治疗。AgNPs 的治疗以剂量依赖的方式恢复了血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、乳酸脱氢酶（LDH）、碱性磷酸酶（ALP）、胆红素、甘油三酯（TG）和胆固醇的水平。在 AgNPs 处理的动物的肝脏中，发现脂质过氧化（LPO）显著减少，还原型谷胱甘肽（GSH）得到恢复。由于 APAP 诱导的肝毒性，肝脏中三磷酸腺苷酶（ATPase）、葡萄糖-6-磷酸酶（G6Pase）和抗氧化酶（如超氧化物歧化酶[SOD]和过氧化氢酶[CAT]）的活性降低，AgNPs 的治疗恢复了这些酶的活性。肝组织形态学的改善也与生化观察结果一致。结果表明，AgNPs 对 APAP 诱导的肝损伤表现出显著的剂量依赖性保护作用。
