Richardson J J R, Hendrickse C, Gao-Smith F, Thickett D R
Institute of Inflammation and Ageing, College of Medical and Dental Sciences, The University of Birmingham, Birmingham B15 2TT, UK.
Department of Colorectal Surgery, Heart of England NHS Foundation Trust, Birmingham B9 5SS, UK.
Int J Inflam. 2017;2017:4915062. doi: 10.1155/2017/4915062. Epub 2017 Jul 30.
Neutrophil Extracellular Traps (NETs) are extracellular neutrophil derived DNA webs which have been implicated in cancer progression and in the development of metastases. NETs production in patients with colorectal cancer was investigated to elucidate their role and prognostic significance.
Systemic neutrophils were isolated from consecutive patients with colorectal cancer and from age-matched healthy volunteers. Neutrophils were stimulated to produce NETs which were quantified by a measure of the fluorescence of the extracellular DNA. The impact of cancer location, tumour stage, and patient outcomes (complications, length of stay, and mortality) on NET production was investigated.
Quantification of NET formation was performed in patients with colorectal cancer ( = 45) and in well-matched healthy individuals ( = 20). Significant increases in NETs production in response to no stimulant (9,735 AFU versus 11347 AFU, = 0.0209), IL-8 (8,644 AFU versus 11,915 AFU, = 0.0032), and LPS (10,576 AFU versus 12,473 AFU, = 0.0428) were identified in patients with colorectal cancer. A significant increase in NETs production in response to fMLP was detected in patients who developed significant postoperative complications (11,760 AFU versus 18,340 AFU, = 0.0242) and who had a prolonged hospital recovery (9,008 AFU versus 12,530 AFU, = 0.0476). An increase in NETs production was also observed in patients who died, but this did not reach statistical significance. Cancer location and tumour stage did not appear to affect preoperative NETs production.
Patients with colorectal cancer have significantly increased NETs production in vitro when compared to healthy volunteers, possibly implicating them in cancer development. Adverse patient outcomes were associated with increased preoperative NETs production, which highlights them as potential therapeutic targets.
中性粒细胞胞外诱捕网（NETs）是源自中性粒细胞的细胞外DNA网络，与癌症进展和转移的发生有关。本研究旨在探讨结直肠癌患者NETs的产生情况，以阐明其作用和预后意义。
从连续的结直肠癌患者和年龄匹配的健康志愿者中分离出全身中性粒细胞。刺激中性粒细胞产生NETs，并通过测量细胞外DNA的荧光进行定量。研究癌症位置、肿瘤分期和患者预后（并发症、住院时间和死亡率）对NETs产生的影响。
对45例结直肠癌患者和20例匹配良好的健康个体进行了NETs形成的定量分析。结果发现，与健康个体相比，结直肠癌患者在无刺激物（9735任意荧光单位对11347任意荧光单位，P = 0.0209）、白细胞介素-8（8644任意荧光单位对119
