Research Laboratory for Pediatric Hemato-Oncology, Department of Pediatric Hemato-Oncology, Dana Children's Hospital, Tel Aviv Sourasky Medical Center Tel Aviv, Israel.
Front Immunol. 2013 Mar 6;4:48. doi: 10.3389/fimmu.2013.00048. eCollection 2013.
Upon activation, neutrophils release fibers composed of chromatin and neutrophil proteins termed neutrophil extracellular traps (NETs). NETs trap and kill microbes, activate dendritic cells and T cells, and are implicated in autoimmune and vascular diseases. Given the growing interest in the role of neutrophils in cancer immunoediting and the diverse function of NETs, we searched for NETs release by tumor-associated neutrophils (TANs). Using pediatric Ewing sarcoma (ES) as a model, we retrospectively examined histopathological material from diagnostic biopsies of eight patients (mean ± SD age of 11.5 ± 4.7 years). TANs were found in six patients and in two of those we identified NETs. These two patients presented with metastatic disease and despite entering complete remission after intensive chemotherapy had an early relapse. NETs were not identified in the diagnostic biopsies of two patients with localized disease and two with metastatic disease. This study is the first to show that TANs in ES are activated to make NETs, pointing to a possible role of NETs in cancer.
中性粒细胞被激活后会释放由染色质和中性粒细胞蛋白组成的纤维，称为中性粒细胞胞外诱捕网（NETs）。NETs 可以捕获和杀死微生物，激活树突状细胞和 T 细胞，并与自身免疫性疾病和血管疾病有关。鉴于人们对中性粒细胞在癌症免疫编辑中的作用以及 NETs 的多种功能越来越感兴趣，我们寻找了肿瘤相关中性粒细胞（TANs）释放的 NETs。我们使用儿科尤文肉瘤（ES）作为模型，回顾性地检查了来自 8 名患者（平均年龄 ± 标准差为 11.5 ± 4.7 岁）诊断性活检的组织病理学材料。在 6 名患者中发现了 TANs，在其中 2 名患者中鉴定到了 NETs。这两名患者患有转移性疾病，尽管在强化化疗后进入完全缓解期，但仍早期复发。在局部疾病和转移性疾病的另外 2 名患者的诊断性活检中未鉴定到 NETs。这项研究首次表明，ES 中的 TANs 被激活形成 NETs，这表明 NETs 在癌症中可能发挥作用。
