Masuda H, Cande W Z
Cell. 1987 Apr 24;49(2):193-202. doi: 10.1016/0092-8674(87)90560-5.
We describe the effect of exogenous tubulin on reactivation of anaphase spindle elongation in isolated diatom spindles. In the absence of tubulin, spindle elongation is limited to the equivalent of the microtubule overlap zone, but in the presence of tubulin spindle elongation is several times the length of the overlap zone. Biotinylated neurotubulin is incorporated into the overlap zone and around the poles. Before spindles have elongated by the equivalent of the overlap zone, there are two regions of incorporated tubulin flanking this zone. After further elongation, there is one broad zone of incorporated tubulin in the spindle midzone. Spindle elongation and the pattern of tubulin incorporation into the midzone, but not the poles, are ATP-dependent and vanadate-sensitive. These results suggest that tubulin adds onto the ends of microtubules in the overlap zone, which then slide through the midzone as the spindle elongates.
我们描述了外源性微管蛋白对分离的硅藻纺锤体后期纺锤体伸长重新激活的影响。在没有微管蛋白的情况下，纺锤体伸长仅限于相当于微管重叠区的长度，但在有微管蛋白的情况下，纺锤体伸长是重叠区长度的几倍。生物素化的神经微管蛋白掺入重叠区和两极周围。在纺锤体伸长到相当于重叠区的长度之前，有两个掺入微管蛋白的区域位于该区域两侧。进一步伸长后，在纺锤体中区有一个宽阔的掺入微管蛋白的区域。纺锤体伸长以及微管蛋白掺入中区而非两极的模式是ATP依赖性的且对钒酸盐敏感。这些结果表明，微管蛋白添加到重叠区微管的末端，然后随着纺锤体伸长而滑过中区。
