Hahn Martin W, Karbon Gerlinde, Koll Ulrike, Schmidt Johanna, Lang Elke
Research Institute for Limnology, University of Innsbruck, Mondseestrasse 9, A-5310 Mondsee, Austria.
Leibniz-Institut DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3261-3267. doi: 10.1099/ijsem.0.002096. Epub 2017 Aug 22.
Strain MWH-Weng1-1T, isolated from an acidic freshwater habitat located in the Wenger Moor, Austria, was characterized by investigating its phenotypic, chemotaxonomic and genomic traits. Phylogenetic analyses based on 16S rRNA gene sequencing placed the strain in the cryptic species complex PnecC within the genus Polynucleobacter. The strain had a genome of 2.04 Mbp with a G+C content of 45.6 mol%. The major fatty acids of the strain were C16 : 1ω7c, C16 : 0 and C18 : 1ω7c. In order to resolve the systematic position of the strain within the species complex PnecC, concatenated partial sequences of eight housekeeping genes were used for phylogenetic analyses. The obtained trees did not place strain MWH-Weng1-1T close to any of the six previously described species within this cryptic species complex. Pairwise whole genome average nucleotide identity comparisons with genome sequences of strains representing the six previously described species of the subcluster resulted throughout in values <78 %, which clearly suggested that strain MWH-Weng1-1T (DSM 24018T=CIP 111099T) represents a novel species. We propose the name Polynucleobacter sphagniphilus sp. nov. and strain MWH-Weng1-1T as the type strain for this new species.
从奥地利温格沼泽地的酸性淡水生境中分离出的菌株MWH-Weng1-1T，通过对其表型、化学分类学和基因组特征进行研究来进行表征。基于16S rRNA基因测序的系统发育分析将该菌株置于多核杆菌属内的隐匿种复合体PnecC中。该菌株的基因组大小为2.04 Mbp，G+C含量为45.6 mol%。该菌株的主要脂肪酸为C16 : 1ω7c、C16 : 0和C18 : 1ω7c。为了确定该菌株在物种复合体PnecC中的系统位置，使用了8个管家基因的串联部分序列进行系统发育分析。所得树状图并未将菌株MWH-Weng1-1T置于该隐匿种复合体内任何六个先前描述的物种附近。与代表该亚群六个先前描述物种的菌株基因组序列进行的全基因组平均核苷酸同一性成对比较结果始终低于78 %，这清楚地表明菌株MWH-Weng1-1T（DSM 24018T=CIP 111099T）代表一个新物种。我们提议将该新物种命名为嗜泥炭多核杆菌（Polynucleobacter sphagniphilus sp. nov.），并将菌株MWH-Weng1-1T作为该新物种的模式菌株。
