Hahn Martin W, Huymann Lesley R, Koll Ulrike, Schmidt Johanna, Lang Elke, Hoetzinger Matthias
Research Institute for Limnology, University of Innsbruck, Mondseestrasse 9, Mondsee A-5310, Austria.
DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, D-38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2017 Feb;67(2):379-385. doi: 10.1099/ijsem.0.001637. Epub 2017 Mar 16.
Strain QLW-P1FAT50C-4T, isolated from a shallow, acidic freshwater pond located in the Austrian Alps at an altitude of 1300 m, was characterized by investigation of phenotypic, chemotaxonomic and genomic traits. As shown previously, phylogenetic analyses based on 16S rRNA gene sequences placed the strain in the cryptic species complex PnecC within the genus Polynucleobacter. The major fatty acids of the strain were C16 : 1ω7c and C18 : 1ω7c. The strain has a genome of 2.23 Mbp with a DNA G+C content of 44.9 mol%. The strain encodes a seemingly complete gene cluster for anoxygenic photosynthesis but lacks typical genes for CO2 assimilation. In order to resolve the phylogenetic position of the strain within the species complex PnecC, concatenated partial sequences of eight housekeeping genes were analysed. The phylogenetic reconstruction obtained did not place strain QLW-P1FAT50C-4T close to any of the five previously described species within subcluster PnecC. Pairwise average nucleotide identity (ANI) comparisons of whole-genome sequences suggested that strain QLW-P1FAT50C-4T (=DSM 24008T=CIP 111100T) represents a novel species, for which we propose the name Polynucleobacter wuianus sp. nov.
菌株QLW-P1FAT50C-4T是从奥地利阿尔卑斯山海拔1300米处一个浅的酸性淡水池塘中分离得到的，通过对其表型、化学分类学和基因组特征进行研究来对其进行表征。如前所示，基于16S rRNA基因序列的系统发育分析将该菌株置于多核杆菌属内的隐秘物种复合体PnecC中。该菌株的主要脂肪酸为C16 : 1ω7c和C18 : 1ω7c。该菌株基因组大小为2.23 Mbp，DNA G+C含量为44.9 mol%。该菌株编码一个看似完整的无氧光合作用基因簇，但缺乏典型的二氧化碳同化基因。为了确定该菌株在物种复合体PnecC中的系统发育位置，对8个管家基因的串联部分序列进行了分析。所获得的系统发育重建结果并未将菌株QLW-P1FAT50C-4T置于PnecC亚群中之前描述的5个物种中的任何一个附近。全基因组序列的成对平均核苷酸同一性（ANI）比较表明，菌株QLW-P1FAT50C-4T（=DSM 24008T=CIP 111100T）代表一个新物种，我们为此提议将其命名为吴氏多核杆菌（Polynucleobacter wuianus sp. nov.）。
