Huang Ke, Xu Yan, Zhang Jun, Chen Chuan, Gao Fan, Zhao Fang-Jie
Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK.
Int J Syst Evol Microbiol. 2017 Sep;67(9):3186-3191. doi: 10.1099/ijsem.0.002068. Epub 2017 Aug 22.
A novel bacterium with strong arsenic (As) methylation and volatilization abilities, designated strain SM-1T, was isolated from an As-contaminated paddy soil. SM-1T is strictly aerobic, rod-shaped, non-motile, Gram-negative and orange-coloured. Phylogenetic analysis revealed that strain SM-1T showed low 16S rRNA gene sequence similarities (≤88 %) to members of established genera in the family Cytophagaceae. Growth of this strain was observed at 15-45 °C (optimum, 37 °C), pH 6.0-8.0 (optimum, 7.0) and 0-0.5 % (w/v) NaCl. The major cellular fatty acids were C16 : 1ω5c and iso-C15 : 0. The respiratory quinone was MK-7, and the predominant polar lipids were phosphatidylethanolamine, an unidentified lipid (L), and an unidentified aminolipid (AL2). The DNA G+C content was 51.5 mol%. On the basis of phenotypic, phylogenetic and chemotaxonomic properties, strain SM-1T represents a novel species in a new genus within the family Cytophagaceae, for which the name Arsenicibacter rosenii gen. nov., sp. nov. is proposed. The type strain of Arsenicibacter rosenii is SM-1T (=CCTCC AB 2017086T=KCTC 52624T).
从砷污染的稻田土壤中分离出一种具有很强砷甲基化和挥发能力的新型细菌，命名为菌株SM-1T。SM-1T严格需氧，杆状，无运动性，革兰氏阴性，呈橙色。系统发育分析表明，菌株SM-1T与噬纤维菌科已确立属的成员的16S rRNA基因序列相似性较低（≤88%）。该菌株在15-45°C（最适温度37°C）、pH 6.0-8.0（最适pH 7.0）和0-0.5%（w/v）NaCl条件下生长。主要细胞脂肪酸为C16:1ω5c和异-C15:0。呼吸醌为MK-7，主要极性脂为磷脂酰乙醇胺、一种未鉴定的脂类（L）和一种未鉴定的氨基脂类（AL2）。DNA G+C含量为51.5 mol%。基于表型、系统发育和化学分类学特性，菌株SM-1T代表噬纤维菌科一个新属中的一个新物种，为此提出新属名为砷杆菌属（Arsenicibacter），新种名为罗森砷杆菌（Arsenicibacter rosenii）。罗森砷杆菌的模式菌株为SM-1T（=CCTCC AB 2017086T=KCTC 52624T）。
