Jiang Fan, Danzeng Wangmu, Zhang Yuming, Zhang Yan, Jiang Li, Liu Jia, Lu Lu, Fan Wei, Peng Fang
Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Shenzhen 518120, PR China.
China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2018 Feb;68(2):663-668. doi: 10.1099/ijsem.0.002563.
A red-pigmented, Gram-reaction-negative, aerobic, non-motile and rod-shaped bacterium, designated NY03-3-30, was isolated from a soil sample collected from Inexpressible Island, Northern Victoria Land of the Antarctic Ross Orogen, and subjected to a polyphasic taxonomic study. Growth occurred at 4-28 °C (optimum 20 °C) and at pH 6.0-9.0 (optimum pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NY03-3-30 belonged to the genus Hymenobacter in the family Cytophagaceae. 16S rRNA gene sequence similarities between strain NY03-3-30 and the type strains of Hymenobacter species with validly published names ranged from 92.7 to 96.2 %. Strain NY03-3-30 contained summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, C16 : 0, C16 : 1ω5c, anteiso-C15 : 0 and summed feature 4 (iso-C17 : 1-I and/or anteiso-C17 : 1-B) as major cellular fatty acids, MK-7 as the respiratory quinone and phosphatidylethanolamine as the main polar lipid. The DNA G+C content of strain NY03-3-30 was 59.4 mol%. On the basis of phylogenetic, physiological and chemotaxonomic data, strain NY03-3-30 is considered to represent a novel species of genus Hymenobacter, for which the name Hymenobacter rubripertinctus sp. nov. is proposed. The type strain is NY03-3-30 (=CCTCC AB 2017095=KCTC 62163).
从南极罗斯造山带维多利亚地北部难以言表岛采集的土壤样本中分离出一株红色色素沉着、革兰氏阴性、需氧、不运动的杆状细菌，命名为NY03-3-30，并对其进行了多相分类研究。该菌在4-28°C（最适温度20°C）和pH 6.0-9.0（最适pH 7.0）条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株NY03-3-30属于噬细胞菌科的Hymenobacter属。菌株NY03-3-30与已有效发表名称的Hymenobacter属模式菌株之间的16S rRNA基因序列相似性在92.7%至96.2%之间。菌株NY03-3-30主要的细胞脂肪酸包括特征性总和3（C16:1ω7c和/或C16:1ω6c）、异-C15:0、C16:0、C16:1ω5c、anteiso-C15:0和特征性总和4（异-C17:1-I和/或anteiso-C17:1-B），呼吸醌为MK-7，主要极性脂为磷脂酰乙醇胺。菌株NY03-3-30的DNA G+C含量为59.4 mol%。基于系统发育、生理和化学分类学数据，菌株NY03-3-30被认为代表Hymenobacter属的一个新物种，为此提出新名称Hymenobacter rubripertinctus sp. nov.。模式菌株为NY03-3-30（=CCTCC AB 2017095=KCTC 62163）。
