Ten Leonid N, Okiria Joseph, Lee Jae-Jin, Lee Seung-Yeol, Kang In-Kyu, Lee Dae Sung, Jung Hee-Young
School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
Department of Horticultural Science, Kyungpook National University, Daegu 41566, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5198-5204. doi: 10.1099/ijsem.0.002440. Epub 2017 Oct 23.
A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterium, designated 15J8-5, was isolated from beach soil on Jeju Island, Republic of Korea. The isolate grew at 10-37 °C (optimal 25 °C), pH 6-8 (optimal pH 7) and in the presence of 0-1 % (w/v) NaCl. The results of comparative 16S rRNA gene sequence analysis indicated that strain 15J8-5 represented a member of the family Cytophagaceae, phylum Bacteroidetes and was most closely related to Spirosoma knui 15J8-12 (93.1 % similarity), Spirosoma spitsbergense SPM-9 (93.1 %) and Spirosoma endophyticum EX36 (93.1 %). The G+C content of the genomic DNA of the novel strain was 48.0 mol%. The isolate contained menaquinone MK-7 as the predominant respiratory quinone, phosphatidylethanolamine as the major polar lipid, and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c; 32.8 %), C16 : 1ω5c (24.8 %) and C16 : 0 (11.7 %) as the major fatty acids, which supported the affiliation of strain 15J8-5 to the genus Spirosoma. The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 15J8-5 from members of the genus Spirosoma. Based on its phenotypic properties and phylogenetic distinctiveness, strain 15J8-5 represents a novel species of the genus Spirosoma, for which the name Spirosomakoreense sp. nov. is proposed. The type strain is 15J8-5 (=KCTC 52027=JCM 31992).
从韩国济州岛的海滩土壤中分离出一株革兰氏染色阴性、无运动性、不形成芽孢、杆状的需氧细菌，命名为15J8 - 5。该菌株在10 - 37°C（最适温度25°C）、pH 6 - 8（最适pH 7）以及0 - 1%（w/v）NaCl存在的条件下生长。比较16S rRNA基因序列分析结果表明，菌株15J8 - 5属于拟杆菌门噬细胞菌科，与克氏螺旋体菌15J8 - 12（相似度93.1%）、斯匹次卑尔根螺旋体菌SPM - 9（93.1%）和内生螺旋体菌EX36（93.1%）关系最为密切。该新菌株基因组DNA的G + C含量为48.0 mol%。该菌株以甲基萘醌MK - 7作为主要呼吸醌，以磷脂酰乙醇胺作为主要极性脂质，以总和特征3（C16 : 1ω7c/C16 : 1ω6c；32.8%）、C16 : 1ω5c（24.8%）和C16 : 0（11.7%）作为主要脂肪酸成分，这些结果支持菌株15J8 - 5归属于螺旋体菌属。生理生化测试结果使菌株15J8 - 5能够与螺旋体菌属的其他成员进行基因型和表型区分。基于其表型特性和系统发育独特性，菌株15J8 - 5代表螺旋体菌属的一个新物种，为此提出新名称韩国螺旋体菌（Spirosomakoreense sp. nov.）。模式菌株为15J8 - 5（=KCTC 52027=JCM 31992）。
