Hilmy A M, el-Domiaty N A, Wershana K
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1987;86(2):247-53. doi: 10.1016/0742-8413(87)90075-2.
The present study is an attempt to define acute (96 hr) and chronic (6-months) effects of nitrite on two different sizes of juvenile Clarias lazera. Static bioassays on acute toxicity showed median tolerance limit (TLm) for four days of 28 and 32 mg/l NO2-N for the two sizes respectively. Although nitrite is known to cause hypoxia, as a result of oxidation of haemoglobin to methemoglobin, exposure of both sizes to nitrite resulted in a decreased erythrocyte count haemoglobin content and haematocrit values. Fish exposed to nitrite responded physiologically by producing methemoglobin. A decline in serum total protein levels was recorded during acute and chronic exposure of both sizes to nitrite. This was interpreted as a generalized stress response.
本研究旨在确定亚硝酸盐对两种不同大小的幼年非洲胡鲶的急性（96小时）和慢性（6个月）影响。急性毒性的静态生物测定表明，两种大小的鱼在四天内的半数耐受限度（TLm）分别为28毫克/升和32毫克/升的亚硝酸盐氮。尽管已知亚硝酸盐会由于血红蛋白氧化为高铁血红蛋白而导致缺氧，但两种大小的鱼暴露于亚硝酸盐中均导致红细胞计数、血红蛋白含量和血细胞比容值下降。暴露于亚硝酸盐的鱼通过产生高铁血红蛋白做出生理反应。在两种大小的鱼急性和慢性暴露于亚硝酸盐期间，均记录到血清总蛋白水平下降。这被解释为一种全身性应激反应。
