Gréen K
Acta Obstet Gynecol Scand Suppl. 1979;87:15-20.
The biosynthesis of prostaglandins is a very rapid reaction. Since blood platelets, when disturbed, readily convert e.g. arachidonic acid into prostaglandin E2 and F2 alpha, those compounds cannot be quantitated in body fluids or tissues which contain platelets. When present in the circulation, primary prostaglandins are rapidly inactivated to corresponding 15-keto-13, 14-dihydro metabolites which occur in plasma in about ten times higher concentrations than the primary prostaglandins. The basal plasma levels of these metabolites are lower than 100 picog/ml. Therefore highly sensitive and specific techniques are necessary for safe quantitation of prostaglandins. The rapid inactivation of prostaglandins also makes it necessary to carefully determine which metabolite should be quantitated in a given experimental situation. The complexity of prostaglandin biochemistry and its importance for accurate monitoring of prostaglandin synthesis in different experimental situations will be discussed in this article.
前列腺素的生物合成是一个非常快速的反应。由于血小板在受到干扰时会轻易地将例如花生四烯酸转化为前列腺素E2和F2α，因此在含有血小板的体液或组织中无法对这些化合物进行定量。当存在于循环系统中时，初级前列腺素会迅速失活为相应的15-酮-13,14-二氢代谢产物，这些代谢产物在血浆中的浓度大约是初级前列腺素的十倍。这些代谢产物的基础血浆水平低于100皮克/毫升。因此，为了安全地定量前列腺素，需要高度灵敏和特异的技术。前列腺素的快速失活也使得有必要仔细确定在给定的实验情况下应该定量哪种代谢产物。本文将讨论前列腺素生物化学的复杂性及其在不同实验情况下对准确监测前列腺素合成的重要性。
