State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, P. R. China.
Nanoscale. 2017 Aug 31;9(34):12746-12754. doi: 10.1039/c7nr04241h.
We report the facile synthesis of polyaniline (PANI)-loaded γ-polyglutamic acid (γ-PGA) nanogels (NGs) for photoacoustic (PA) imaging-guided photothermal therapy (PTT) of tumors. In this work, γ-PGA NGs were first formed via a double emulsion approach, followed by crosslinking with cystamine dihydrochloride (Cys) via 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride coupling chemistry. The formed γ-PGA/Cys NGs were employed as a nanoreactor to load aniline monomers via an electrostatic interaction for subsequent in situ polymerization in the presence of ammonium persulfate. The resulting γ-PGA/Cys@PANI NGs were thoroughly characterized. It is shown that the γ-PGA/Cys@PANI NGs with an average size of 71.9 nm are dispersible in water, colloidally stable, and cytocompatible and hemocompatible in the concentration range studied. The strong near-infrared (NIR) absorbance renders the NGs with good PA imaging contrast enhancement and photothermal conversion properties. With these excellent properties and biocompatibility, the developed γ-PGA/Cys@PANI NGs are able to be used for PA imaging-guided PTT of cancer cells in vitro and a xenografted tumor model in vivo. This unique theranostic nanoplatform may be further loaded with other imaging or therapeutic elements, or modified with targeting ligands, thereby providing a ubiquitous platform for multimode imaging and combinational therapy of different biosystems.
我们报告了一种简便的方法，用于合成负载聚丙氨酸（PANI）的γ-聚谷氨酸（γ-PGA）纳米凝胶（NG），用于肿瘤的光声（PA）成像引导光热治疗（PTT）。在这项工作中，首先通过双重乳液法形成 γ-PGA NGs，然后通过 1-乙基-3-[3-二甲基氨基丙基]碳化二亚胺盐酸盐偶联化学与胱胺二盐酸盐（Cys）交联。形成的 γ-PGA/Cys NGs 被用作纳米反应器，通过静电相互作用负载苯胺单体，然后在过硫酸铵存在下进行原位聚合。对所得的 γ-PGA/Cys@PANI NGs 进行了彻底的表征。结果表明，平均粒径为 71.9nm 的 γ-PGA/Cys@PANI NGs 在水中具有良好的分散性，胶体稳定性，并且在所研究的浓度范围内具有细胞相容性和血液相容性。强大的近红外（NIR）吸收使 NGs 具有良好的 PA 成像对比度增强和光热转换性能。由于具有这些优异的性能和生物相容性，开发的 γ-PGA/Cys@PANI NGs 可用于体外癌细胞的 PA 成像引导 PTT 和体内异种移植肿瘤模型。这种独特的治疗性纳米平台可以进一步负载其他成像或治疗元素，或修饰靶向配体，从而为不同生物系统的多模式成像和组合治疗提供通用平台。
